Development and cross-species amplification of 18 microsatellite markers in the Sumatran tiger (Panthera tigris sumatrae)

J. E. Williamson; R. M. Huebinger; J. A. Sommer; E. E. Louis; R. C. Barber

doi:10.1046/j.1471-8286.2002.00163.x

Development and cross-species amplification of 18 microsatellite markers in the Sumatran tiger (Panthera tigris sumatrae)

J. E. Williamson, R. M. Huebinger, J. A. Sommer, E. E. Louis, R. C. Barber

Institute for Healthy Aging

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21 Scopus citations

Abstract

Eighteen polymorphic microsatellite loci from the highly endangered Sumatran tiger (Panthera tigris sumatrae) were isolated and characterized. Upon polymerase chain reaction amplification, 16 of these markers produced a single, sharp band in all three tiger and 10 non-tiger felid species examined. Of the two remaining loci, 6HDZ057 and 6HDZ635 failed to amplify genomic DNA from puma (Felis concolor) and cheetah (Acinonyx jubatus), respectively. The amplification of these markers across four genera is an indication of their usefulness for population genetics studies and conservation work in a wide range of felid species.

Original language	English
Pages (from-to)	110-112
Number of pages	3
Journal	Molecular Ecology Notes
Volume	2
Issue number	2
DOIs	https://doi.org/10.1046/j.1471-8286.2002.00163.x
State	Published - 2002

Keywords

Cross-amplification
Felidae
Genetic markers
Microsatellites
Tiger

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10.1046/j.1471-8286.2002.00163.x

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title = "Development and cross-species amplification of 18 microsatellite markers in the Sumatran tiger (Panthera tigris sumatrae)",

abstract = "Eighteen polymorphic microsatellite loci from the highly endangered Sumatran tiger (Panthera tigris sumatrae) were isolated and characterized. Upon polymerase chain reaction amplification, 16 of these markers produced a single, sharp band in all three tiger and 10 non-tiger felid species examined. Of the two remaining loci, 6HDZ057 and 6HDZ635 failed to amplify genomic DNA from puma (Felis concolor) and cheetah (Acinonyx jubatus), respectively. The amplification of these markers across four genera is an indication of their usefulness for population genetics studies and conservation work in a wide range of felid species.",

keywords = "Cross-amplification, Felidae, Genetic markers, Microsatellites, Tiger",

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AU - Sommer, J. A.

AU - Louis, E. E.

AU - Barber, R. C.

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AB - Eighteen polymorphic microsatellite loci from the highly endangered Sumatran tiger (Panthera tigris sumatrae) were isolated and characterized. Upon polymerase chain reaction amplification, 16 of these markers produced a single, sharp band in all three tiger and 10 non-tiger felid species examined. Of the two remaining loci, 6HDZ057 and 6HDZ635 failed to amplify genomic DNA from puma (Felis concolor) and cheetah (Acinonyx jubatus), respectively. The amplification of these markers across four genera is an indication of their usefulness for population genetics studies and conservation work in a wide range of felid species.

KW - Cross-amplification

KW - Felidae

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Development and cross-species amplification of 18 microsatellite markers in the Sumatran tiger (Panthera tigris sumatrae)

Abstract

Keywords

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