TY - JOUR
T1 - Development and characterization of two mini-X chromosomal short tandem repeat multiplexes
AU - Diegoli, Toni M.
AU - Coble, Michael D.
PY - 2011/11
Y1 - 2011/11
N2 - This study presents the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes utilizing reduced-size amplicons (less than 200 base pairs) for identity and kinship testing with degraded DNA. Approximately 1360 samples across 4 U.S. population groups were typed for 15 X chromosomal STR markers: DXS6789, DXS7130, DXS9902, GATA31E08, DXS7424, GATA165B12, DXS101, DXS6795, GATA172D05, DXS10147, DXS8378, DXS7132, DXS6803, HPRTB, and DXS7423. A high degree of polymorphism was observed for each marker and both multiplexes were sensitive down to 200 pg of pristine DNA. The two proposed multiplexes are suitable for forensic use, and show potential for improved analysis of compromised bone samples.
AB - This study presents the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes utilizing reduced-size amplicons (less than 200 base pairs) for identity and kinship testing with degraded DNA. Approximately 1360 samples across 4 U.S. population groups were typed for 15 X chromosomal STR markers: DXS6789, DXS7130, DXS9902, GATA31E08, DXS7424, GATA165B12, DXS101, DXS6795, GATA172D05, DXS10147, DXS8378, DXS7132, DXS6803, HPRTB, and DXS7423. A high degree of polymorphism was observed for each marker and both multiplexes were sensitive down to 200 pg of pristine DNA. The two proposed multiplexes are suitable for forensic use, and show potential for improved analysis of compromised bone samples.
KW - Identity testing
KW - Kinship testing
KW - Mini-STRs
KW - Multiplex PCR
KW - U.S. population
KW - X chromosome
UR - http://www.scopus.com/inward/record.url?scp=80052263626&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2010.08.019
DO - 10.1016/j.fsigen.2010.08.019
M3 - Article
C2 - 20943451
AN - SCOPUS:80052263626
SN - 1872-4973
VL - 5
SP - 415
EP - 421
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
IS - 5
ER -