Development and characterization of two mini-X chromosomal short tandem repeat multiplexes

Toni M. Diegoli, Michael D. Coble

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

This study presents the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes utilizing reduced-size amplicons (less than 200 base pairs) for identity and kinship testing with degraded DNA. Approximately 1360 samples across 4 U.S. population groups were typed for 15 X chromosomal STR markers: DXS6789, DXS7130, DXS9902, GATA31E08, DXS7424, GATA165B12, DXS101, DXS6795, GATA172D05, DXS10147, DXS8378, DXS7132, DXS6803, HPRTB, and DXS7423. A high degree of polymorphism was observed for each marker and both multiplexes were sensitive down to 200 pg of pristine DNA. The two proposed multiplexes are suitable for forensic use, and show potential for improved analysis of compromised bone samples.

Original languageEnglish
Pages (from-to)415-421
Number of pages7
JournalForensic Science International: Genetics
Volume5
Issue number5
DOIs
StatePublished - 1 Nov 2011

Keywords

  • Identity testing
  • Kinship testing
  • Mini-STRs
  • Multiplex PCR
  • U.S. population
  • X chromosome

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