Development and characterization of a recombinant cDNA-based hepatitis C virus system

Jean Ndjomou; Ying Liu; John O'Malley; Maria Ericsson; Johnny Jianglin He

doi:10.1016/j.bbrc.2007.05.073

Development and characterization of a recombinant cDNA-based hepatitis C virus system

Jean Ndjomou, Ying Liu, John O'Malley, Maria Ericsson, Johnny Jianglin He

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Abstract

Invention of subgenomic HCV replicon a few years ago and recent success of in vitro production of infectious HCV have improved our knowledge of the HCV life cycle, replication, pathogenesis, and screening of anti-HCV therapeutics. However, the highly genotype-dependent nature of the in vitro HCV production system has limited its potential for HCV research. In this study, we constructed a recombinant DNA-based HCV system that contained EF-1α promoter-driven HCV genotype 1b with HCV E1/E2 deleted and replaced by GFP. We co-transfected this recombinant cDNA with HCV E1/E2 or VSV-G expression plasmid into 293T cells, and we showed HCV protein expression and processing and demonstrated production of HCV-like particles in culture supernatant of co-transfected cells. These results support potential use of this system for studies on expression and processing of the HCV polyprotein and assembly and release of HCV-like particles.

Original language	English
Pages (from-to)	57-62
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	359
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2007.05.073
State	Published - 20 Jul 2007

Keywords

293T cells
HCV
Recombinant system
Virus-like particles

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10.1016/j.bbrc.2007.05.073

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abstract = "Invention of subgenomic HCV replicon a few years ago and recent success of in vitro production of infectious HCV have improved our knowledge of the HCV life cycle, replication, pathogenesis, and screening of anti-HCV therapeutics. However, the highly genotype-dependent nature of the in vitro HCV production system has limited its potential for HCV research. In this study, we constructed a recombinant DNA-based HCV system that contained EF-1α promoter-driven HCV genotype 1b with HCV E1/E2 deleted and replaced by GFP. We co-transfected this recombinant cDNA with HCV E1/E2 or VSV-G expression plasmid into 293T cells, and we showed HCV protein expression and processing and demonstrated production of HCV-like particles in culture supernatant of co-transfected cells. These results support potential use of this system for studies on expression and processing of the HCV polyprotein and assembly and release of HCV-like particles.",

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AU - Ndjomou, Jean

AU - Liu, Ying

AU - O'Malley, John

AU - Ericsson, Maria

AU - He, Johnny Jianglin

PY - 2007/7/20

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AB - Invention of subgenomic HCV replicon a few years ago and recent success of in vitro production of infectious HCV have improved our knowledge of the HCV life cycle, replication, pathogenesis, and screening of anti-HCV therapeutics. However, the highly genotype-dependent nature of the in vitro HCV production system has limited its potential for HCV research. In this study, we constructed a recombinant DNA-based HCV system that contained EF-1α promoter-driven HCV genotype 1b with HCV E1/E2 deleted and replaced by GFP. We co-transfected this recombinant cDNA with HCV E1/E2 or VSV-G expression plasmid into 293T cells, and we showed HCV protein expression and processing and demonstrated production of HCV-like particles in culture supernatant of co-transfected cells. These results support potential use of this system for studies on expression and processing of the HCV polyprotein and assembly and release of HCV-like particles.

KW - 293T cells

KW - HCV

KW - Recombinant system

KW - Virus-like particles

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Development and characterization of a recombinant cDNA-based hepatitis C virus system

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