Development and characterization of a recombinant cDNA-based hepatitis C virus system

Jean Ndjomou, Ying Liu, John O'Malley, Maria Ericsson, Johnny Jianglin He

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Invention of subgenomic HCV replicon a few years ago and recent success of in vitro production of infectious HCV have improved our knowledge of the HCV life cycle, replication, pathogenesis, and screening of anti-HCV therapeutics. However, the highly genotype-dependent nature of the in vitro HCV production system has limited its potential for HCV research. In this study, we constructed a recombinant DNA-based HCV system that contained EF-1α promoter-driven HCV genotype 1b with HCV E1/E2 deleted and replaced by GFP. We co-transfected this recombinant cDNA with HCV E1/E2 or VSV-G expression plasmid into 293T cells, and we showed HCV protein expression and processing and demonstrated production of HCV-like particles in culture supernatant of co-transfected cells. These results support potential use of this system for studies on expression and processing of the HCV polyprotein and assembly and release of HCV-like particles.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume359
Issue number1
DOIs
StatePublished - 20 Jul 2007

Keywords

  • 293T cells
  • HCV
  • Recombinant system
  • Virus-like particles

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