Abstract
Invention of subgenomic HCV replicon a few years ago and recent success of in vitro production of infectious HCV have improved our knowledge of the HCV life cycle, replication, pathogenesis, and screening of anti-HCV therapeutics. However, the highly genotype-dependent nature of the in vitro HCV production system has limited its potential for HCV research. In this study, we constructed a recombinant DNA-based HCV system that contained EF-1α promoter-driven HCV genotype 1b with HCV E1/E2 deleted and replaced by GFP. We co-transfected this recombinant cDNA with HCV E1/E2 or VSV-G expression plasmid into 293T cells, and we showed HCV protein expression and processing and demonstrated production of HCV-like particles in culture supernatant of co-transfected cells. These results support potential use of this system for studies on expression and processing of the HCV polyprotein and assembly and release of HCV-like particles.
Original language | English |
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Pages (from-to) | 57-62 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 359 |
Issue number | 1 |
DOIs | |
State | Published - 20 Jul 2007 |
Keywords
- 293T cells
- HCV
- Recombinant system
- Virus-like particles