Environmental metals are potentially toxic and can interfere with the metal-binding motifs of various critical proteins. This unit describes an electrophoretic method that can be used to measure the ability of a xenobiotic metal to bind a zinc-finger motif. Information gained using this protocol can lead to the identification of protein targets of metals and shed better light on their mechanisms of action.
|Journal||Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]|
|State||Published - Feb 2004|