Determination of thailandepsin B in rat plasma with a LC-MS/MS method

Zhongzhe Cheng, Lian Ding, Yang Zhang, Guiying Chen, Chang Chen, Yi Qiang Cheng, Hongliang Jiang

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background: Thailandepsin B (TDP-B) is a potent histone deacetylase inhibitor under development. A reliable bioanalytical method for the quantification of TDP-B in plasma samples is required. Results: The stabilizer mixture containing hydrochloric acid, formic acid and dichlorvos was applied to stabilize TDP-B in matrix samples. The method validation was conducted over the curve range of 1.00 to 1000 ng/ml. The intrabatch and interbatch precision and accuracy of the quality control samples showed ≤10.9% relative standard deviation and -6.7% to 15.0% relative error. Moreover, a possible metabolite M of TDP-B was tentatively characterized. Conclusion: A reliable LC-MS/MS method was developed and validated for the quantification of TDP-B and was successfully applied to a rat pharmacokinetic study.

Original languageEnglish
Pages (from-to)39-52
Number of pages14
JournalBioanalysis
Volume7
Issue number1
DOIs
StatePublished - 1 Jan 2015

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    Cheng, Z., Ding, L., Zhang, Y., Chen, G., Chen, C., Cheng, Y. Q., & Jiang, H. (2015). Determination of thailandepsin B in rat plasma with a LC-MS/MS method. Bioanalysis, 7(1), 39-52. https://doi.org/10.4155/bio.14.264