Abstract
A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.
Original language | English |
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Pages (from-to) | 168-173 |
Number of pages | 6 |
Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
Volume | 613 |
Issue number | 1 |
DOIs | |
State | Published - 5 Mar 1993 |