Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection

Ming Cheng Chung; Shih Ku Lin; Wen Ho Chang; Michael W. Jann

doi:10.1016/0378-4347(93)80212-M

Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection

Ming Cheng Chung, Shih Ku Lin, Wen Ho Chang, Michael W. Jann

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.

Original language	English
Pages (from-to)	168-173
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	613
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(93)80212-M
State	Published - 5 Mar 1993

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title = "Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection",

abstract = "A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.",

author = "Chung, {Ming Cheng} and Lin, {Shih Ku} and Chang, {Wen Ho} and Jann, {Michael W.}",

note = "Funding Information: This work was supported by Grant NCS 8 l-0412-B109-501fr om the National ScienceC oun- cil and the Taipei City Government.T he authors thank Ms. Lee-Hua Huang and Su-Chen Chang for technical assistance.",

year = "1993",

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Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection. / Chung, Ming Cheng; Lin, Shih Ku; Chang, Wen Ho; Jann, Michael W.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 613, No. 1, 05.03.1993, p. 168-173.

Research output: Contribution to journal › Article › peer-review

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T1 - Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection

AU - Chung, Ming Cheng

AU - Lin, Shih Ku

AU - Chang, Wen Ho

AU - Jann, Michael W.

N1 - Funding Information: This work was supported by Grant NCS 8 l-0412-B109-501fr om the National ScienceC oun- cil and the Taipei City Government.T he authors thank Ms. Lee-Hua Huang and Su-Chen Chang for technical assistance.

PY - 1993/3/5

Y1 - 1993/3/5

N2 - A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.

AB - A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.

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Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection

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