Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection

Ming Cheng Chung, Shih Ku Lin, Wen Ho Chang, Michael W. Jann

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.

Original languageEnglish
Pages (from-to)168-173
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume613
Issue number1
DOIs
StatePublished - 5 Mar 1993

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