Detection of three rotational correlation times for a rigid asymmetric molecule using frequency-domain fluorometry

Ignacy Gryczynski, Henryk Cherek Joseph R. Lakowicz

Research output: Contribution to journalArticle

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Abstract

We measured the frequency response of the polarized emission of Yt-base in propylene glycol at 10 ° C. Data were obtained for excitation wavelengths of 290, 312 and 346 nm, for which the fundamental anisotropies are 0.05, 0.19 and 0.32, respectively. Additionally, data were obtained using CCl4, to decrease the mean decay time from 9.1 to 4.2 ns. These nine sets of data were analyzed globally to recover the anisotropy decay law. Three correlation times were needed to fit the data, 0.8, 3.0 and 5.6 ns, a range of only 7-fold. We believe this is the first reported detection of three correlation times for a rigid molecule.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalBiophysical Chemistry
Volume30
Issue number3
DOIs
Publication statusPublished - 15 Jul 1988

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Keywords

  • Anisotropy decay
  • Fluorescence
  • Frequency-domain fluorometry
  • Polarization
  • Rotational diffusion

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