TY - JOUR
T1 - Detection of three rotational correlation times for a rigid asymmetric molecule using frequency-domain fluorometry
AU - Gryczynski, Ignacy
AU - Cherek Joseph R. Lakowicz, Henryk
N1 - Funding Information:
This work was supported by grants DMB-8502835 and DMB-8511065 from the National Science Foundation, and grant GM 39617 from the National Institutes of Health.
PY - 1988/7/15
Y1 - 1988/7/15
N2 - We measured the frequency response of the polarized emission of Yt-base in propylene glycol at 10 ° C. Data were obtained for excitation wavelengths of 290, 312 and 346 nm, for which the fundamental anisotropies are 0.05, 0.19 and 0.32, respectively. Additionally, data were obtained using CCl4, to decrease the mean decay time from 9.1 to 4.2 ns. These nine sets of data were analyzed globally to recover the anisotropy decay law. Three correlation times were needed to fit the data, 0.8, 3.0 and 5.6 ns, a range of only 7-fold. We believe this is the first reported detection of three correlation times for a rigid molecule.
AB - We measured the frequency response of the polarized emission of Yt-base in propylene glycol at 10 ° C. Data were obtained for excitation wavelengths of 290, 312 and 346 nm, for which the fundamental anisotropies are 0.05, 0.19 and 0.32, respectively. Additionally, data were obtained using CCl4, to decrease the mean decay time from 9.1 to 4.2 ns. These nine sets of data were analyzed globally to recover the anisotropy decay law. Three correlation times were needed to fit the data, 0.8, 3.0 and 5.6 ns, a range of only 7-fold. We believe this is the first reported detection of three correlation times for a rigid molecule.
KW - Anisotropy decay
KW - Fluorescence
KW - Frequency-domain fluorometry
KW - Polarization
KW - Rotational diffusion
UR - http://www.scopus.com/inward/record.url?scp=0024288295&partnerID=8YFLogxK
U2 - 10.1016/0301-4622(88)85022-1
DO - 10.1016/0301-4622(88)85022-1
M3 - Article
C2 - 3207846
AN - SCOPUS:0024288295
SN - 0301-4622
VL - 30
SP - 271
EP - 277
JO - Biophysical Chemistry
JF - Biophysical Chemistry
IS - 3
ER -