Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction

Charles Thill, P. Bryon Backenson, Melissa A. Prusinski, Sarah J. Kogut, Joon Hak Lee, James L. Coleman

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

Original languageEnglish
Pages (from-to)694-696
Number of pages3
JournalJournal of Medical Entomology
Volume42
Issue number4
DOIs
StatePublished - Jul 2005

Keywords

  • Amblyomma
  • Babesia
  • Chelex
  • Detection
  • Ixodcs

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