Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction

Charles Thill; P. Bryon Backenson; Melissa A. Prusinski; Sarah J. Kogut; Joon Hak Lee; James L. Coleman

doi:10.1093/jmedent/42.4.694

Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction

Charles Thill, P. Bryon Backenson, Melissa A. Prusinski, Sarah J. Kogut, Joon Hak Lee, James L. Coleman

Biostatistics & Epidemiology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

Original language	English
Pages (from-to)	694-696
Number of pages	3
Journal	Journal of Medical Entomology
Volume	42
Issue number	4
DOIs	https://doi.org/10.1093/jmedent/42.4.694
State	Published - Jul 2005

Keywords

Amblyomma
Babesia
Chelex
Detection
Ixodcs

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10.1093/jmedent/42.4.694

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title = "Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction",

abstract = "A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.",

keywords = "Amblyomma, Babesia, Chelex, Detection, Ixodcs",

author = "Charles Thill and Backenson, {P. Bryon} and Prusinski, {Melissa A.} and Kogut, {Sarah J.} and Lee, {Joon Hak} and Coleman, {James L.}",

year = "2005",

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language = "English",

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pages = "694--696",

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AU - Thill, Charles

AU - Backenson, P. Bryon

AU - Prusinski, Melissa A.

AU - Kogut, Sarah J.

AU - Lee, Joon Hak

AU - Coleman, James L.

PY - 2005/7

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N2 - A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

AB - A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

KW - Amblyomma

KW - Babesia

KW - Chelex

KW - Detection

KW - Ixodcs

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DO - 10.1093/jmedent/42.4.694

M3 - Article

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AN - SCOPUS:22844435434

SN - 0022-2585

VL - 42

SP - 694

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ER -

Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction

Abstract

Keywords

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