Abstract
We report a data-dependent neutral-loss-driven MS3 acquisition to enhance, in addition to abundant Michael adducts, the detection of Schiff-base adducts of proteins and 4-hydroxy-2-nonenal, a reactive end product of lipid peroxidation. In vitro modification of cytochrome c oxidase, a mitochondrial protein complex, was used as a model to evaluate the method. The technique allowed for a confident validation of modification sites and also identified a Schiff-base adduct in subunit Vb of the protein complex.
Original language | English |
---|---|
Pages (from-to) | 5188-5193 |
Number of pages | 6 |
Journal | Proteomics |
Volume | 9 |
Issue number | 22 |
DOIs | |
State | Published - Nov 2009 |
Keywords
- 4-Hydroxy-2-nonenal
- Michael addition
- Neutral loss-driven MS
- Protein adducts
- Schiff base
- Technology