Detection and identification of 4-hydroxy-2-nonenal Schiff-base adducts along with products of Michael addition using data-dependent neutral loss-driven MS3 acquisition: Method evaluation through an in vitro study on cytochrome c oxidase modifications

Navin Rauniyar, Laszlo Prokai

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We report a data-dependent neutral-loss-driven MS3 acquisition to enhance, in addition to abundant Michael adducts, the detection of Schiff-base adducts of proteins and 4-hydroxy-2-nonenal, a reactive end product of lipid peroxidation. In vitro modification of cytochrome c oxidase, a mitochondrial protein complex, was used as a model to evaluate the method. The technique allowed for a confident validation of modification sites and also identified a Schiff-base adduct in subunit Vb of the protein complex.

Original languageEnglish
Pages (from-to)5188-5193
Number of pages6
JournalProteomics
Volume9
Issue number22
DOIs
StatePublished - 1 Nov 2009

Keywords

  • 4-Hydroxy-2-nonenal
  • Michael addition
  • Neutral loss-driven MS
  • Protein adducts
  • Schiff base
  • Technology

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