TY - JOUR
T1 - Description of the Sphingolipid Content and Subspecies in the Diabetic Cornea
AU - Priyadarsini, Shrestha
AU - Sarker-Nag, Akhee
AU - Allegood, Jeremy
AU - Chalfant, Charles
AU - Karamichos, DImitrios
N1 - Funding Information:
The authors declare that there is no conflict of interest. This work was supported by research grants from the Veteran’s Administration (VA Merit Review I BX001792 (CEC) and a Research Career Scientist Award 13F-RCS-002(CEC)); from the National Institutes of Health via HL125353(CEC), CA154314 (C.E.C),EY020886 (D.K), EY023568 (D.K), and NH1C06-RR17393 (to Virginia Commonwealth University for renovation); from unrestricted grant from Research to Prevent Blindness (New York, NY, USA). Services and products in support of the research project were generated by the VCU Massey Cancer Center Shared supported, in part, with funding from NIH-NCI Cancer Center Support Grant P30 CA016059. The contents of this manuscript do not represent the views of the Department of Veterans Affairs or the United States Government.
Funding Information:
The authors declare that there is no conflict of interest. This work was supported by research grants from the Veteran’s Administration (VA Merit Review I BX001792 (CEC) and a Research Career Scientist Award 13F-RCS-002(CEC)); from the National Institutes of Health via HL125353(CEC), CA154314 (C.E.C),EY020886 (D.K), EY023568 (D.K), and NH1C06-RR17393 (to Virginia Commonwealth University for renovation); from unrestricted grant from Research to Prevent Blindness (New York, NY, USA). Services and products in support of the research project were generated by the VCU Massey Cancer Center Shared supported, in part, with funding from NIH-NCI Cancer Center Support Grant P30 CA016059. The contents of this manuscript do not represent the views of the Department of Veterans Affairs or the United States Government.
Publisher Copyright:
© 2015 Taylor & Francis Group, LLC.
PY - 2015/12/2
Y1 - 2015/12/2
N2 - Purpose: Diabetes mellitus (DM) is characterized by high blood sugar levels over a prolonged period. Long term complications include but not limited heart disease, stroke, kidney failure, and ocular damage. An estimated 382 million people are diagnosed with Type 2 DM accounting for 90% of the cases. Common corneal dysfunctions associated with DM result in impaired vision due to decreased wound healing, corneal edema, and altered epithelial basement membrane. Lipids play a fundamental role in tissue metabolism and disease states. We attempt to determine the role of sphingolipids (SPL) in human Type I and Type II diabetic corneas.Materials and Methods: Cadaver corneas from healthy (non-diabetic/no ocular trauma), Type I (T1DM), and Type II diabetic (T2DM) donors were obtained and processed for lipidomics using LC-MS/MS.Results: Our data show significant differences in the SPL composition between control, T1DM and T2DM corneas. Both T1DM and T2DM showed a 10-folddownregulation of sphingomyelin(SM), 5-fold up regulation of Ceramides (Cer) and 2-fold upregulation of monohexosylceramides (MHC). Differences were also seen in total amounts of SPL where Cer was increased by approximately 3 fold in both T1DM and T2DM where SM decreased by 50% in both T1DM and T2DM when compared to healthy controls. No differences were seen in MHC amounts.Conclusions: Overall, our data indicate major differences in SPL distribution in human diabetic corneas. Information on the sphingolipids role in cornea, corneal cell physiology, and diseases are very limitedwhich highlights the importance of these findings.
AB - Purpose: Diabetes mellitus (DM) is characterized by high blood sugar levels over a prolonged period. Long term complications include but not limited heart disease, stroke, kidney failure, and ocular damage. An estimated 382 million people are diagnosed with Type 2 DM accounting for 90% of the cases. Common corneal dysfunctions associated with DM result in impaired vision due to decreased wound healing, corneal edema, and altered epithelial basement membrane. Lipids play a fundamental role in tissue metabolism and disease states. We attempt to determine the role of sphingolipids (SPL) in human Type I and Type II diabetic corneas.Materials and Methods: Cadaver corneas from healthy (non-diabetic/no ocular trauma), Type I (T1DM), and Type II diabetic (T2DM) donors were obtained and processed for lipidomics using LC-MS/MS.Results: Our data show significant differences in the SPL composition between control, T1DM and T2DM corneas. Both T1DM and T2DM showed a 10-folddownregulation of sphingomyelin(SM), 5-fold up regulation of Ceramides (Cer) and 2-fold upregulation of monohexosylceramides (MHC). Differences were also seen in total amounts of SPL where Cer was increased by approximately 3 fold in both T1DM and T2DM where SM decreased by 50% in both T1DM and T2DM when compared to healthy controls. No differences were seen in MHC amounts.Conclusions: Overall, our data indicate major differences in SPL distribution in human diabetic corneas. Information on the sphingolipids role in cornea, corneal cell physiology, and diseases are very limitedwhich highlights the importance of these findings.
KW - Cornea
KW - Diabetes
KW - Lipidomics
KW - Sphingolipids
UR - http://www.scopus.com/inward/record.url?scp=84959524537&partnerID=8YFLogxK
U2 - 10.3109/02713683.2014.990984
DO - 10.3109/02713683.2014.990984
M3 - Article
C2 - 25426847
AN - SCOPUS:84959524537
SN - 0271-3683
VL - 40
SP - 1204
EP - 1210
JO - Current Eye Research
JF - Current Eye Research
IS - 12
ER -