TY - JOUR
T1 - Dengue seroprevalence and seroconversion in urban and rural populations in northeastern thailand and southern laos
AU - Doum, Dyna
AU - Overgaard, Hans J.
AU - Mayxay, Mayfong
AU - Suttiprapa, Sutas
AU - Saichua, Prasert
AU - Ekalaksananan, Tipaya
AU - Tongchai, Panwad
AU - Rahman, Md Siddikur
AU - Haque, Ubydul
AU - Phommachanh, Sysavanh
AU - Pongvongsa, Tiengkham
AU - Rocklöv, Joacim
AU - Paul, Richard
AU - Pientong, Chamsai
N1 - Funding Information:
Acknowledgments: The authors would like to thank the Research Council of Norway and the Khon Kaen University Faculty of Medicine research grant for financial support for this study. Also acknowledged to the Thai Royal Scholarship under Her Royal Highness Princess Maha Chakri Sirindhorn Education Project for providing a scholarship to pursue a Ph.D. at the Khon Kaen University in Thailand. Moreover, we thank Nanthasane Vannavong for project administration and furnishing the resources to implement the study. Finally, sincere thanks to the project teams, local authorities, fieldworkers, volunteers in both countries for all the assistance and Sumaira Zafar for created a map for the study sites.
Funding Information:
Funding: The project was funded by the Research Council of Norway (project no. 281077) and Khon Kaen University Faculty of Medicine Research Grant (no. IN63223).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Dengue is the most rapidly spreading mosquito-borne viral disease in the world. The detection of clinical cases enables us to measure the incidence of dengue infection, whereas serological surveys give insights into the prevalence of infection. This study aimed to determine dengue seroprevalence and seroconversion rates in northeastern Thailand and southern Laos and to assess any association of mosquito control methods and socioeconomic factors with dengue virus (DENV) infection. Cross-sectional seroprevalence surveys were performed in May and November 2019 on the same individuals. Blood samples were collected from one adult and one child, when possible, in each of 720 randomly selected households from two urban and two rural sites in both northeastern Thailand and southern Laos. IgG antibodies against DENV were detected in serum using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Overall, 1071 individuals participated in the study. The seroprevalence rate was high (91.5%) across all 8 study sites. Only age and province were associated with seroprevalence rates. There were 33 seroconversions during the period from May to November, of which seven reported fever. More than half of the seroconversions occurred in the rural areas and in Laos. Dengue seroconversion was significantly associated with young age (<15 years old), female gender, province, and duration of living in the current residence. No socioeconomic factors or mosquito control methods were found to be associated with seroprevalence or seroconversion. Notably, however, the province with most seroconversions had lower diurnal temperature ranges than elsewhere. In conclusion, our study has highlighted the homogeneity of dengue exposure across a wide range of settings and most notably those from rural and urban areas. Dengue can no longer be considered to be solely an urban disease nor necessarily one linked to poverty.
AB - Dengue is the most rapidly spreading mosquito-borne viral disease in the world. The detection of clinical cases enables us to measure the incidence of dengue infection, whereas serological surveys give insights into the prevalence of infection. This study aimed to determine dengue seroprevalence and seroconversion rates in northeastern Thailand and southern Laos and to assess any association of mosquito control methods and socioeconomic factors with dengue virus (DENV) infection. Cross-sectional seroprevalence surveys were performed in May and November 2019 on the same individuals. Blood samples were collected from one adult and one child, when possible, in each of 720 randomly selected households from two urban and two rural sites in both northeastern Thailand and southern Laos. IgG antibodies against DENV were detected in serum using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Overall, 1071 individuals participated in the study. The seroprevalence rate was high (91.5%) across all 8 study sites. Only age and province were associated with seroprevalence rates. There were 33 seroconversions during the period from May to November, of which seven reported fever. More than half of the seroconversions occurred in the rural areas and in Laos. Dengue seroconversion was significantly associated with young age (<15 years old), female gender, province, and duration of living in the current residence. No socioeconomic factors or mosquito control methods were found to be associated with seroprevalence or seroconversion. Notably, however, the province with most seroconversions had lower diurnal temperature ranges than elsewhere. In conclusion, our study has highlighted the homogeneity of dengue exposure across a wide range of settings and most notably those from rural and urban areas. Dengue can no longer be considered to be solely an urban disease nor necessarily one linked to poverty.
KW - DENV
KW - Dengue
KW - Seroconversion
KW - Seroprevalence
KW - Socioeconomic factors
UR - http://www.scopus.com/inward/record.url?scp=85097365476&partnerID=8YFLogxK
U2 - 10.3390/ijerph17239134
DO - 10.3390/ijerph17239134
M3 - Article
C2 - 33297445
AN - SCOPUS:85097365476
SN - 1661-7827
VL - 17
SP - 1
EP - 16
JO - International journal of environmental research and public health
JF - International journal of environmental research and public health
IS - 23
M1 - 9134
ER -