Abstract
We measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101 (R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.
| Original language | English |
|---|---|
| Article number | 015001 |
| Journal | Methods and Applications in Fluorescence |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Mar 2016 |
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Keywords
- 3D FRET
- FRET in solution
- Fluorescence
- Forster distance
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Demonstration of FRET in solutions. / Shah, Sunil; Gryczynski, Zygmunt; Chib, Rahul; Fudala, Rafal; Baxi, Aatmun; Borejdo, Julian; Synak, Anna; Gryczynski, Ignacy.
In: Methods and Applications in Fluorescence, Vol. 4, No. 1, 015001, 01.03.2016.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Demonstration of FRET in solutions
AU - Shah, Sunil
AU - Gryczynski, Zygmunt
AU - Chib, Rahul
AU - Fudala, Rafal
AU - Baxi, Aatmun
AU - Borejdo, Julian
AU - Synak, Anna
AU - Gryczynski, Ignacy
PY - 2016/3/1
Y1 - 2016/3/1
N2 - We measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101 (R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.
AB - We measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101 (R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.
KW - 3D FRET
KW - FRET in solution
KW - Fluorescence
KW - Forster distance
UR - http://www.scopus.com/inward/record.url?scp=84974623696&partnerID=8YFLogxK
U2 - 10.1088/2050-6120/4/1/015001
DO - 10.1088/2050-6120/4/1/015001
M3 - Article
VL - 4
JO - Methods and applications in fluorescence
JF - Methods and applications in fluorescence
SN - 2050-6120
IS - 1
M1 - 015001
ER -