Demonstration of FRET in solutions

Sunil Shah, Zygmunt Gryczynski, Rahul Chib, Rafal Fudala, Aatmun Baxi, Julian Borejdo, Anna Synak, Ignacy Gryczynski

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

We measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101 (R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.

Original languageEnglish
Article number015001
JournalMethods and Applications in Fluorescence
Volume4
Issue number1
DOIs
StatePublished - 1 Mar 2016

Fingerprint

Rhodamines
rhodamine
Energy transfer
Demonstrations
Fluorescence
energy transfer
fluorescence
Fluorophores
life (durability)
Wavelength
Propylene Glycol
Glycols
propylene
Excited states
wavelengths
Propylene
glycols
Anisotropy
occurrences
Color

Keywords

  • 3D FRET
  • FRET in solution
  • Fluorescence
  • Forster distance

Cite this

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title = "Demonstration of FRET in solutions",
abstract = "We measured the F{\"o}rster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101 (R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.",
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language = "English",
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Demonstration of FRET in solutions. / Shah, Sunil; Gryczynski, Zygmunt; Chib, Rahul; Fudala, Rafal; Baxi, Aatmun; Borejdo, Julian; Synak, Anna; Gryczynski, Ignacy.

In: Methods and Applications in Fluorescence, Vol. 4, No. 1, 015001, 01.03.2016.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Demonstration of FRET in solutions

AU - Shah, Sunil

AU - Gryczynski, Zygmunt

AU - Chib, Rahul

AU - Fudala, Rafal

AU - Baxi, Aatmun

AU - Borejdo, Julian

AU - Synak, Anna

AU - Gryczynski, Ignacy

PY - 2016/3/1

Y1 - 2016/3/1

N2 - We measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101 (R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.

AB - We measured the Förster resonance energy transfer (FRET) from Uranin (U) donor to Rhodamine 101 (R101) acceptor in propylene glycol. Steady-state fluorescence measurements show a significant difference between mixed and unmixed fluorophore solutions. In the solution with mixed fluorophores, fluorescence intensity of the U donor decreases and intensity of R101 fluorescence increases. This is visualized as a color change from green to orange. Fluorescence anisotropy of the mixture solution increases in the donor emission wavelength region and decreases in the acceptor emission wavelengths; which is consistent with FRET occurrence. Time-resolved (lifetime) measurements show a decrease of the U lifetime in the presence of R101 acceptor. In the intensity decay of R101 acceptor appears a negative component indicating excited state process. All these measurements prove the presence of FRET in U/R101 mixture fluorescence.

KW - 3D FRET

KW - FRET in solution

KW - Fluorescence

KW - Forster distance

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DO - 10.1088/2050-6120/4/1/015001

M3 - Article

VL - 4

JO - Methods and applications in fluorescence

JF - Methods and applications in fluorescence

SN - 2050-6120

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