TY - JOUR
T1 - Degradation and detoxification of hexachlorocyclohexane isomers by Pseudomonas aeruginosa ITRC-5
AU - Chaudhary, Pankaj
AU - Kumar, Manish
AU - Khangarot, Bhom S.
AU - Kumar, Ashwani
N1 - Funding Information:
This work was supported by a grant from Department of Biotechnology, Ministry of Science and Technology, Government of India.
PY - 2006/3
Y1 - 2006/3
N2 - Technical-hexachlorocyclohexane (t-HCH) consists of four major isomers i.e. α-, β-, γ- and δ-HCH. The insecticidal γ-HCH is separated from it by solvent extraction, and the remaining 'muck' is discarded. HCH-isomers from the 'muck' can potentially enter the environment and impart toxicity. For its biological treatment, biodegradation of HCH-isomers by the isolated bacterium Pseudomonas aeruginosa ITRC-5 was evaluated. In its presence, from 1.7 mM 'muck', >98% α- and >80% β-, γ- and δ-HCH were degraded after 24 days of incubation. The degradation was optimal at 1.7 mM input concentration of 'muck', pH 9.0, and temperature 20-30°C. Under these conditions, from 2 g 'muck', >90% Σ-HCH, i.e. the sum of α, β, γ, and δ-HCH, were degraded in a 1.0-liter batch-reactor after incubation for 4 cycles of 5 weeks each. The degradation was accompanied with 90% reduction in the toxicity of 'muck' to the aquatic test organism Daphnia magna. The isolated bacterium ITRC-5 can therefore be used for the degradation and detoxification of HCH-wastes, prior to their disposal.
AB - Technical-hexachlorocyclohexane (t-HCH) consists of four major isomers i.e. α-, β-, γ- and δ-HCH. The insecticidal γ-HCH is separated from it by solvent extraction, and the remaining 'muck' is discarded. HCH-isomers from the 'muck' can potentially enter the environment and impart toxicity. For its biological treatment, biodegradation of HCH-isomers by the isolated bacterium Pseudomonas aeruginosa ITRC-5 was evaluated. In its presence, from 1.7 mM 'muck', >98% α- and >80% β-, γ- and δ-HCH were degraded after 24 days of incubation. The degradation was optimal at 1.7 mM input concentration of 'muck', pH 9.0, and temperature 20-30°C. Under these conditions, from 2 g 'muck', >90% Σ-HCH, i.e. the sum of α, β, γ, and δ-HCH, were degraded in a 1.0-liter batch-reactor after incubation for 4 cycles of 5 weeks each. The degradation was accompanied with 90% reduction in the toxicity of 'muck' to the aquatic test organism Daphnia magna. The isolated bacterium ITRC-5 can therefore be used for the degradation and detoxification of HCH-wastes, prior to their disposal.
KW - Degradation
KW - Detoxification
KW - Hexachlorocyclohexane
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=33644696455&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2005.12.003
DO - 10.1016/j.ibiod.2005.12.003
M3 - Article
AN - SCOPUS:33644696455
SN - 0964-8305
VL - 57
SP - 107
EP - 113
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 2
ER -