Decreasing photobleaching by silver nanoparticles on metal surfaces: Application to muscle myofibrils

Priya Muthu, Ignacy Gryczynski, Zygmunt Gryczynski, John M. Talent, Irina Akopova, Julian Borejdo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recently it has become possible to study single protein molecules in a cell. However, such experiments are plagued by rapid photobleaching. We recently showed that the interaction of fluorophores with localized surface plasmon polaritons (LSPs) induced in the metallic nanoparticles led to a substantial reduction of photobleaching. We now investigate whether the photobleaching could be further reduced when the excited fluorophore interacts with the LSP excited in the metallic nanoparticles resident on mirrored surface. As an example we use myofibrils, subcellular structures within skeletal muscle. We compare nanoparticle-enhanced fluorescence of myofibrils in the presence and in the absence of a mirrored surface. The proximity of the mirrored surface led to enhancement of fluorescence and to a decrease in fluorescent lifetime, much greater than that observed in the presence of nanoparticles alone. We think that the effect is caused by the near-field interactions between fluorophores and LSP, and between fluorophores and propagating surface plasmons (PSPs) produced in the metallic surface by the nanoparticles. Photobleaching is decreased because fluorescence enhancement enables illumination with a weaker laser beam and because the decrease in fluorescence lifetime minimizes the probability of oxygen attack during the time a molecule is in the exited state.

Original languageEnglish
Article number014023
JournalJournal of Biomedical Optics
Volume13
Issue number1
DOIs
StatePublished - 1 Dec 2008

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Photobleaching
muscles
Silver
metal surfaces
Muscle
Metals
silver
Nanoparticles
nanoparticles
Fluorophores
Fluorescence
polaritons
fluorescence
skeletal muscle
life (durability)
Molecules
Plasmons
augmentation
plasmons
attack

Keywords

  • photobleaching
  • surface plasmons

Cite this

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abstract = "Recently it has become possible to study single protein molecules in a cell. However, such experiments are plagued by rapid photobleaching. We recently showed that the interaction of fluorophores with localized surface plasmon polaritons (LSPs) induced in the metallic nanoparticles led to a substantial reduction of photobleaching. We now investigate whether the photobleaching could be further reduced when the excited fluorophore interacts with the LSP excited in the metallic nanoparticles resident on mirrored surface. As an example we use myofibrils, subcellular structures within skeletal muscle. We compare nanoparticle-enhanced fluorescence of myofibrils in the presence and in the absence of a mirrored surface. The proximity of the mirrored surface led to enhancement of fluorescence and to a decrease in fluorescent lifetime, much greater than that observed in the presence of nanoparticles alone. We think that the effect is caused by the near-field interactions between fluorophores and LSP, and between fluorophores and propagating surface plasmons (PSPs) produced in the metallic surface by the nanoparticles. Photobleaching is decreased because fluorescence enhancement enables illumination with a weaker laser beam and because the decrease in fluorescence lifetime minimizes the probability of oxygen attack during the time a molecule is in the exited state.",
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Decreasing photobleaching by silver nanoparticles on metal surfaces : Application to muscle myofibrils. / Muthu, Priya; Gryczynski, Ignacy; Gryczynski, Zygmunt; Talent, John M.; Akopova, Irina; Borejdo, Julian.

In: Journal of Biomedical Optics, Vol. 13, No. 1, 014023, 01.12.2008.

Research output: Contribution to journalArticle

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T2 - Application to muscle myofibrils

AU - Muthu, Priya

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

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AU - Akopova, Irina

AU - Borejdo, Julian

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