Decay time distribution analysis of Yt-base in benzene-methanol mixtures

Ignacy Gryczynski, Wieslaw Wiczk, Joseph R. Lakowicz, Michael L. Johnson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Frequency-domain fluorometry was used to measure intensity decays of synthetic Yt-base in mixtures of benzene-methanol at 20 °C. Multiexponential analysis shows that the decay of Yt-base fluorescence in benzene and methanol can be well fitted to a single-exponential model with τ = 9.67 ns and 6.25 ns respectively. In mixtures of benzene-methanol the decays became heterogeneous, and the maximum of heterogeneity observed was in a mixture containing 6% methanol. Since we expected a distribution of Yt-base solvation states in the solvent mixtures, and because the decay times of Yt-base are sensitive to solvent, we analyzed the data in terms of decay time distributions. The goodness-of-fit for the unimodal distribution model which has two floating parameters was equivalent to that found using the double exponential model with three floating parameters. The Lorentzian distribution model appears to provide a slightly superior fit relative to the Gaussian distribution model. These results suggest that the intensity decays of solvent-sensitive fluorophores in mixed solvents are described by a distribution of decay times.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalJournal of Photochemistry and Photobiology, B: Biology
Issue number2
StatePublished - Nov 1989


  • Frequency-domain fluorometry
  • Lorentzian distribution.
  • fluorescence decay
  • multiexponential analysis


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