Cytoplasmic localization of the white phase-specific WH11 gene product of Candida albicans

Klaus Schröppel, Srikantha Thyagarajan, Deborah Wessels, Melanie DeCock, Shawn R. Lockhart, David R. Soll

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Cells of Candida albicans WO-1 switch frequently, spontaneously and reversibly between a white and opaque phase. The white-opaque transition involves the regulation of phase-specific genes. In the white budding phase, cells express the white phase-specific gene WH11, which encodes a protein with homology to the heat shock protein Hsp12 of Saccharomyces cerevisiae. A recombinant Wh11 protein has been synthesized, purified to apparent homogeneity and used to generate a rabbit polyclonal antiserum. The antiserum was used to localize the Wh11 protein in white phase cells. Wh11 is distributed throughout the cytoplasm but appears to be excluded from vesicles, plasma membrane and nucleus. An analysis by Western blotting of Wh11 expression in a number of C. albicans strains and related species suggests a correlation between round budding cell shape and expression.

Original languageEnglish
Pages (from-to)2245-2254
Number of pages10
JournalMicrobiology
Volume142
Issue number8
DOIs
StatePublished - 1 Jan 1996

Fingerprint

Candida albicans
Immune Sera
Genes
Cell Shape
Heat-Shock Proteins
Recombinant Proteins
Saccharomyces cerevisiae
Cytoplasm
Proteins
Western Blotting
Cell Membrane
Rabbits

Keywords

  • Bud-hypha transition
  • Candida switching
  • Cell shape
  • Recombinant WH11 protein
  • White-opaque transition

Cite this

Schröppel, Klaus ; Thyagarajan, Srikantha ; Wessels, Deborah ; DeCock, Melanie ; Lockhart, Shawn R. ; Soll, David R. / Cytoplasmic localization of the white phase-specific WH11 gene product of Candida albicans. In: Microbiology. 1996 ; Vol. 142, No. 8. pp. 2245-2254.
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abstract = "Cells of Candida albicans WO-1 switch frequently, spontaneously and reversibly between a white and opaque phase. The white-opaque transition involves the regulation of phase-specific genes. In the white budding phase, cells express the white phase-specific gene WH11, which encodes a protein with homology to the heat shock protein Hsp12 of Saccharomyces cerevisiae. A recombinant Wh11 protein has been synthesized, purified to apparent homogeneity and used to generate a rabbit polyclonal antiserum. The antiserum was used to localize the Wh11 protein in white phase cells. Wh11 is distributed throughout the cytoplasm but appears to be excluded from vesicles, plasma membrane and nucleus. An analysis by Western blotting of Wh11 expression in a number of C. albicans strains and related species suggests a correlation between round budding cell shape and expression.",
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Cytoplasmic localization of the white phase-specific WH11 gene product of Candida albicans. / Schröppel, Klaus; Thyagarajan, Srikantha; Wessels, Deborah; DeCock, Melanie; Lockhart, Shawn R.; Soll, David R.

In: Microbiology, Vol. 142, No. 8, 01.01.1996, p. 2245-2254.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Cytoplasmic localization of the white phase-specific WH11 gene product of Candida albicans

AU - Schröppel, Klaus

AU - Thyagarajan, Srikantha

AU - Wessels, Deborah

AU - DeCock, Melanie

AU - Lockhart, Shawn R.

AU - Soll, David R.

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AB - Cells of Candida albicans WO-1 switch frequently, spontaneously and reversibly between a white and opaque phase. The white-opaque transition involves the regulation of phase-specific genes. In the white budding phase, cells express the white phase-specific gene WH11, which encodes a protein with homology to the heat shock protein Hsp12 of Saccharomyces cerevisiae. A recombinant Wh11 protein has been synthesized, purified to apparent homogeneity and used to generate a rabbit polyclonal antiserum. The antiserum was used to localize the Wh11 protein in white phase cells. Wh11 is distributed throughout the cytoplasm but appears to be excluded from vesicles, plasma membrane and nucleus. An analysis by Western blotting of Wh11 expression in a number of C. albicans strains and related species suggests a correlation between round budding cell shape and expression.

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