Current state-of-art of STR sequencing in forensic genetics

Antonio Alonso, Pedro Alberto Barrio, Petra Müller, Steffi Köcher, Burkhard Berger, Pablo Martin, Martin Bodner, Sascha Willuweit, Walther Parson, Lutz Roewer, Bruce Budowle

Research output: Contribution to journalReview article

21 Scopus citations

Abstract

The current state of validation and implementation strategies of massively parallel sequencing (MPS) technology for the analysis of STR markers for forensic genetics use is described, covering the topics of the current catalog of commercial MPS-STR panels, leading MPS-platforms, and MPS-STR data analysis tools. In addition, the developmental and internal validation studies carried out to date to evaluate reliability, sensitivity, mixture analysis, concordance, and the ability to analyze challenged samples are summarized. The results of various MPS-STR population studies that showed a large number of new STR sequence variants that increase the power of discrimination in several forensically relevant loci are also presented. Finally, various initiatives developed by several international projects and standardization (or guidelines) groups to facilitate application of MPS technology for STR marker analyses are discussed in regard to promoting a standard STR sequence nomenclature, performing population studies to detect sequence variants, and developing a universal system to translate sequence variants into a simple STR nomenclature (numbers and letters) compatible with national STR databases.

Original languageEnglish
Pages (from-to)2655-2668
Number of pages14
JournalElectrophoresis
Volume39
Issue number21
DOIs
StatePublished - Nov 2018

Keywords

  • Capillary electrophoresis
  • Forensic genetics
  • Massively parallel sequencing
  • Short tandem repeats
  • Validation studies

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    Alonso, A., Barrio, P. A., Müller, P., Köcher, S., Berger, B., Martin, P., Bodner, M., Willuweit, S., Parson, W., Roewer, L., & Budowle, B. (2018). Current state-of-art of STR sequencing in forensic genetics. Electrophoresis, 39(21), 2655-2668. https://doi.org/10.1002/elps.201800030