TY - JOUR
T1 - Cross-talk between human neural stem/progenitor cells and peripheral blood mononuclear cells in an allogeneic co-culture model
AU - Zhang, Hongxia
AU - Shao, Bei
AU - Zhuge, Qichuan
AU - Wang, Peng
AU - Zheng, Chengcai
AU - Huang, Weilong
AU - Yang, Chenqi
AU - Wang, Brian
AU - Su, Dong Ming
AU - Jin, Kunlin
N1 - Publisher Copyright:
© 2015 PLOS ONE.
PY - 2015/2/6
Y1 - 2015/2/6
N2 - Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise. However, the underlying mechanisms remain unclear. We, here, focused on the interaction between hNSCs and allogeneic peripheral blood mononuclear cells (PBMCs) in a co-culture model. We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture. We also found that PBMCs, in turn, significantly promote the proliferation and differentiation of hNSCs. Our data suggest that hNSCs cross-talk with immune cells.
AB - Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise. However, the underlying mechanisms remain unclear. We, here, focused on the interaction between hNSCs and allogeneic peripheral blood mononuclear cells (PBMCs) in a co-culture model. We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture. We also found that PBMCs, in turn, significantly promote the proliferation and differentiation of hNSCs. Our data suggest that hNSCs cross-talk with immune cells.
UR - http://www.scopus.com/inward/record.url?scp=84922569428&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0117432
DO - 10.1371/journal.pone.0117432
M3 - Article
C2 - 25658950
AN - SCOPUS:84922569428
SN - 1932-6203
VL - 10
JO - PLoS ONE
JF - PLoS ONE
IS - 2
M1 - e0117432
ER -