Cross-talk between human neural stem/progenitor cells and peripheral blood mononuclear cells in an allogeneic co-culture model

Hongxia Zhang, Bei Shao, Qichuan Zhuge, Peng Wang, Chengcai Zheng, Weilong Huang, Chenqi Yang, Brian Wang, Dong Ming Su, Kunlin Jin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise. However, the underlying mechanisms remain unclear. We, here, focused on the interaction between hNSCs and allogeneic peripheral blood mononuclear cells (PBMCs) in a co-culture model. We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture. We also found that PBMCs, in turn, significantly promote the proliferation and differentiation of hNSCs. Our data suggest that hNSCs cross-talk with immune cells.

Original languageEnglish
Article numbere0117432
JournalPLoS ONE
Volume10
Issue number2
DOIs
StatePublished - 6 Feb 2015

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Neural Stem Cells
coculture
mononuclear leukocytes
Coculture Techniques
Stem cells
Cell culture
stem cells
Blood Cells
Blood
Stem Cells
T-cells
Cytokines
T-Lymphocytes
Somatostatin-Secreting Cells
T-lymphocytes
Regulatory T-Lymphocytes
Cell- and Tissue-Based Therapy
cytokines
Anti-Inflammatory Agents
Transplantation

Cite this

Zhang, Hongxia ; Shao, Bei ; Zhuge, Qichuan ; Wang, Peng ; Zheng, Chengcai ; Huang, Weilong ; Yang, Chenqi ; Wang, Brian ; Su, Dong Ming ; Jin, Kunlin. / Cross-talk between human neural stem/progenitor cells and peripheral blood mononuclear cells in an allogeneic co-culture model. In: PLoS ONE. 2015 ; Vol. 10, No. 2.
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Cross-talk between human neural stem/progenitor cells and peripheral blood mononuclear cells in an allogeneic co-culture model. / Zhang, Hongxia; Shao, Bei; Zhuge, Qichuan; Wang, Peng; Zheng, Chengcai; Huang, Weilong; Yang, Chenqi; Wang, Brian; Su, Dong Ming; Jin, Kunlin.

In: PLoS ONE, Vol. 10, No. 2, e0117432, 06.02.2015.

Research output: Contribution to journalArticle

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AU - Zhang, Hongxia

AU - Shao, Bei

AU - Zhuge, Qichuan

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AU - Zheng, Chengcai

AU - Huang, Weilong

AU - Yang, Chenqi

AU - Wang, Brian

AU - Su, Dong Ming

AU - Jin, Kunlin

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AB - Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise. However, the underlying mechanisms remain unclear. We, here, focused on the interaction between hNSCs and allogeneic peripheral blood mononuclear cells (PBMCs) in a co-culture model. We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture. We also found that PBMCs, in turn, significantly promote the proliferation and differentiation of hNSCs. Our data suggest that hNSCs cross-talk with immune cells.

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