Abstract
Genome editing consists of various technologies that involve precise modification of cellular DNA sequences. It utilizes special engineered nucleases that make specific cuts in DNA, which can result in addition, deletion, or replacement of specific nucleotides as well as modification of gene expression or induction epigenetic changes. Genome editing has been extensively used to treat various diseases, including ocular diseases, and also it is currently being utilized to study gene functions. Here, we summarize basic concepts and tools utilized in various genome editing. We further focus on recent applications of CRISPR–Cas9 in various ocular diseases.
Original language | English |
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Title of host publication | Handbook of Basic and Clinical Ocular Pharmacology and Therapeutics |
Publisher | Elsevier |
Pages | 615-623 |
Number of pages | 9 |
ISBN (Electronic) | 9780128192917 |
ISBN (Print) | 9780128192924 |
DOIs | |
State | Published - 1 Jan 2022 |
Keywords
- CRISPR–Cas9
- DNA
- enzymes
- Genome editing