@article{55760c5869d346b8bc0896fa09373240,
title = "CRISPR based editing of SIV proviral DNA in ART treated non-human primates",
abstract = "Elimination of HIV DNA from infected individuals remains a challenge in medicine. Here, we demonstrate that intravenous inoculation of SIV-infected macaques, a well-accepted non-human primate model of HIV infection, with adeno-associated virus 9 (AAV9)-CRISPR/Cas9 gene editing construct designed for eliminating proviral SIV DNA, leads to broad distribution of editing molecules and precise cleavage and removal of fragments of the integrated proviral DNA from the genome of infected blood cells and tissues known to be viral reservoirs including lymph nodes, spleen, bone marrow, and brain among others. Accordingly, AAV9-CRISPR treatment results in a reduction in the percent of proviral DNA in blood and tissues. These proof-of-concept observations offer a promising step toward the elimination of HIV reservoirs in the clinic.",
author = "Pietro Mancuso and Chen Chen and Rafal Kaminski and Jennifer Gordon and Shuren Liao and Robinson, {Jake A.} and Smith, {Mandy D.} and Hong Liu and Sariyer, {Ilker K.} and Rahsan Sariyer and Peterson, {Tiffany A.} and Martina Donadoni and Williams, {Jaclyn B.} and Summer Siddiqui and Bunnell, {Bruce A.} and Binhua Ling and MacLean, {Andrew G.} and Burdo, {Tricia H.} and Kamel Khalili",
note = "Funding Information: The authors wish to thank past and present members of the Department of Neuroscience and Center for Neurovirology for their support and sharing of ideas and reagents. This work was supported by grant support from NIH: P51OD11104, R01NS104016, R56AI143647, R56AI150772, U42OD024282, U42OD010568, P30MH092177, and a Ruth L. Kirschstein National Research Service Award (5T32OD011124-13). Anti-retroviral drugs were generously provided by ViiV Healthcare (DTG) and Gilead (TDF and FTC). We thank Drs. Michael Farzan and Matt Gardner, Scripps Florida, for the AAV NAb screening. This work is dedicated to the memory of Dr. Tiffany A. Peterson. We are grateful for her contributions to these studies. Publisher Copyright: {\textcopyright} 2020, The Author(s).",
year = "2020",
month = dec,
doi = "10.1038/s41467-020-19821-7",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}