Covalent immobilization of liposomes on plasma functionalized metallic surfaces

S. Mourtas, M. Kastellorizios, P. Klepetsanis, E. Farsari, E. Amanatides, D. Mataras, B. R. Pistillo, P. Favia, E. Sardella, R. d'Agostino, S. G. Antimisiaris

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

A method was developed to functionalize biomedical metals with liposomes. The novelty of the method includes the plasma-functionalization of the metal surface with proper chemical groups to be used as anchor sites for the covalent immobilization of the liposomes. Stainless steel (SS-316) disks were processed in radiofrequency glow discharges fed with vapors of acrylic acid to coat them with thin adherent films characterized by surface carboxylic groups, where liposomes were covalently bound through the formation of amide bonds. For this, liposomes decorated with polyethylene glycol molecules bearing terminal amine-groups were prepared. After ensuring that the liposomes remain intact, under the conditions applying for immobilization; different attachment conditions were evaluated (incubation time, concentration of liposome dispersion) for optimization of the technique. Immobilization of calcein-entrapping liposomes was evaluated by monitoring the percent of calcein attached on the surfaces. Best results were obtained when liposome dispersions with 5. mg/ml (liposomal lipid) concentration were incubated on each disk for 24. h at 37°C. The method is proposed for developing drug-eluting biomedical materials or devices by using liposomes that have appropriate membrane compositions and are loaded with drugs or other bioactive agents.

Original languageEnglish
Pages (from-to)214-220
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume84
Issue number1
DOIs
StatePublished - 1 May 2011

Keywords

  • Controlled release
  • Drug delivery
  • Drug eluting
  • Immobilization
  • Liposomes
  • Plasma processes

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    Mourtas, S., Kastellorizios, M., Klepetsanis, P., Farsari, E., Amanatides, E., Mataras, D., Pistillo, B. R., Favia, P., Sardella, E., d'Agostino, R., & Antimisiaris, S. G. (2011). Covalent immobilization of liposomes on plasma functionalized metallic surfaces. Colloids and Surfaces B: Biointerfaces, 84(1), 214-220. https://doi.org/10.1016/j.colsurfb.2011.01.002