Coupled plasmon effects for the enhancement of fluorescent immunoassays

Anne Barnett, Evgenia G. Matveeva, Ignacy Gryczynski, Zygmunt Gryczynski, Ewa M. Goldys

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We present a study of fluoroimmunoassays using Rhodamine-RedX labelled antibodies enhanced by nanoparticle silver island films (SIFs) in the presence of thin metallic layers. Our results show enhancement of the immunoassay signal by up to 10-fold for SIFs on glass and up to 50-fold enhancement for SIFs supported by a metal surface. An explanation for these effects is proposed by considering plasmonic coupling within the system and modification of the fluorophore spontaneous emission rate.

Original languageEnglish
Pages (from-to)297-300
Number of pages4
JournalPhysica B: Condensed Matter
Volume394
Issue number2
DOIs
StatePublished - 15 May 2007

Fingerprint

immunoassay
Silver
silver
augmentation
Rhodamines
Fluorophores
Spontaneous emission
rhodamine
antibodies
Antibodies
spontaneous emission
metal surfaces
Metals
Nanoparticles
Glass
nanoparticles
glass

Keywords

  • Myoglobin immunoassay
  • Plasmonic coupling
  • Silver island films

Cite this

Barnett, Anne ; Matveeva, Evgenia G. ; Gryczynski, Ignacy ; Gryczynski, Zygmunt ; Goldys, Ewa M. / Coupled plasmon effects for the enhancement of fluorescent immunoassays. In: Physica B: Condensed Matter. 2007 ; Vol. 394, No. 2. pp. 297-300.
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Coupled plasmon effects for the enhancement of fluorescent immunoassays. / Barnett, Anne; Matveeva, Evgenia G.; Gryczynski, Ignacy; Gryczynski, Zygmunt; Goldys, Ewa M.

In: Physica B: Condensed Matter, Vol. 394, No. 2, 15.05.2007, p. 297-300.

Research output: Contribution to journalArticle

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AU - Matveeva, Evgenia G.

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Goldys, Ewa M.

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