Coupled plasmon effects for the enhancement of fluorescent immunoassays

Anne Barnett; Evgenia G. Matveeva; Ignacy Gryczynski; Zygmunt Gryczynski; Ewa M. Goldys

doi:10.1016/j.physb.2006.12.085

Coupled plasmon effects for the enhancement of fluorescent immunoassays

Anne Barnett, Evgenia G. Matveeva, Ignacy Gryczynski, Zygmunt Gryczynski, Ewa M. Goldys

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We present a study of fluoroimmunoassays using Rhodamine-RedX labelled antibodies enhanced by nanoparticle silver island films (SIFs) in the presence of thin metallic layers. Our results show enhancement of the immunoassay signal by up to 10-fold for SIFs on glass and up to 50-fold enhancement for SIFs supported by a metal surface. An explanation for these effects is proposed by considering plasmonic coupling within the system and modification of the fluorophore spontaneous emission rate.

Original language	English
Pages (from-to)	297-300
Number of pages	4
Journal	Physica B: Condensed Matter
Volume	394
Issue number	2
DOIs	https://doi.org/10.1016/j.physb.2006.12.085
State	Published - 15 May 2007

Keywords

Myoglobin immunoassay
Plasmonic coupling
Silver island films

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10.1016/j.physb.2006.12.085

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title = "Coupled plasmon effects for the enhancement of fluorescent immunoassays",

abstract = "We present a study of fluoroimmunoassays using Rhodamine-RedX labelled antibodies enhanced by nanoparticle silver island films (SIFs) in the presence of thin metallic layers. Our results show enhancement of the immunoassay signal by up to 10-fold for SIFs on glass and up to 50-fold enhancement for SIFs supported by a metal surface. An explanation for these effects is proposed by considering plasmonic coupling within the system and modification of the fluorophore spontaneous emission rate.",

keywords = "Myoglobin immunoassay, Plasmonic coupling, Silver island films",

author = "Anne Barnett and Matveeva, {Evgenia G.} and Ignacy Gryczynski and Zygmunt Gryczynski and Goldys, {Ewa M.}",

note = "Funding Information: This work was supported by NIH (NCI CA 114460, NIBIB EB-1690), BITC, and Philip Morris USA, Inc. Results were partially reported at ACS meeting (Atlanta, GA, USA, March 2006). ",

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T1 - Coupled plasmon effects for the enhancement of fluorescent immunoassays

AU - Barnett, Anne

AU - Matveeva, Evgenia G.

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Goldys, Ewa M.

N1 - Funding Information: This work was supported by NIH (NCI CA 114460, NIBIB EB-1690), BITC, and Philip Morris USA, Inc. Results were partially reported at ACS meeting (Atlanta, GA, USA, March 2006).

PY - 2007/5/15

Y1 - 2007/5/15

N2 - We present a study of fluoroimmunoassays using Rhodamine-RedX labelled antibodies enhanced by nanoparticle silver island films (SIFs) in the presence of thin metallic layers. Our results show enhancement of the immunoassay signal by up to 10-fold for SIFs on glass and up to 50-fold enhancement for SIFs supported by a metal surface. An explanation for these effects is proposed by considering plasmonic coupling within the system and modification of the fluorophore spontaneous emission rate.

AB - We present a study of fluoroimmunoassays using Rhodamine-RedX labelled antibodies enhanced by nanoparticle silver island films (SIFs) in the presence of thin metallic layers. Our results show enhancement of the immunoassay signal by up to 10-fold for SIFs on glass and up to 50-fold enhancement for SIFs supported by a metal surface. An explanation for these effects is proposed by considering plasmonic coupling within the system and modification of the fluorophore spontaneous emission rate.

KW - Myoglobin immunoassay

KW - Plasmonic coupling

KW - Silver island films

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DO - 10.1016/j.physb.2006.12.085

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VL - 394

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EP - 300

JO - Physica B: Condensed Matter

JF - Physica B: Condensed Matter

IS - 2

ER -

Coupled plasmon effects for the enhancement of fluorescent immunoassays

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Keywords

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