Coupled plasmon effects for the enhancement of fluorescent immunoassays

Anne Barnett, Evgenia G. Matveeva, Ignacy Gryczynski, Zygmunt Gryczynski, Ewa M. Goldys

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We present a study of fluoroimmunoassays using Rhodamine-RedX labelled antibodies enhanced by nanoparticle silver island films (SIFs) in the presence of thin metallic layers. Our results show enhancement of the immunoassay signal by up to 10-fold for SIFs on glass and up to 50-fold enhancement for SIFs supported by a metal surface. An explanation for these effects is proposed by considering plasmonic coupling within the system and modification of the fluorophore spontaneous emission rate.

Original languageEnglish
Pages (from-to)297-300
Number of pages4
JournalPhysica B: Condensed Matter
Volume394
Issue number2
DOIs
StatePublished - 15 May 2007

Keywords

  • Myoglobin immunoassay
  • Plasmonic coupling
  • Silver island films

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