TY - JOUR
T1 - Correspondence regarding Schwend and Gustafsson, "False positives in MALDI-TOF detection of ERβ in mitochondria"
AU - Yang, Shaohua
AU - Prokai, Laszlo
AU - Simpkins, James W.
PY - 2006/7/7
Y1 - 2006/7/7
N2 - Recently, Schwend and Gustafsson tried to use the MALDI-TOF methods to confirm one of the results reported by Yang et al., which provided definitive evidences to demonstrate the localization of estrogen receptor β (ERβ) in the mitochondria of multiple cell types, using immunocytochemistry, immunoblot, and proteomic approaches. Analysis of the data with the MASCOT database algorithm provided no evidence for the presence of ERβ in the mouse live mitochondria, in which very low ERβ expression has been detected in their own report. On the other hand, our MALDI-TOF analysis using human heart mitochondrial protein has identified 7 and 8 sequences that could be potentially from ERβ and ERβ3, respectively, but not from ATP synthases. Further, none of the sequences identified by us as those of ERβ and ERβ3 shares m/z targeted by Schwend and Gustafsson in their measurements. Therefore, the claim by Gustafsson's laboratory about false positives in MALDI-TOF detection of ERβ in mitochondria has no relevance to our report.
AB - Recently, Schwend and Gustafsson tried to use the MALDI-TOF methods to confirm one of the results reported by Yang et al., which provided definitive evidences to demonstrate the localization of estrogen receptor β (ERβ) in the mitochondria of multiple cell types, using immunocytochemistry, immunoblot, and proteomic approaches. Analysis of the data with the MASCOT database algorithm provided no evidence for the presence of ERβ in the mouse live mitochondria, in which very low ERβ expression has been detected in their own report. On the other hand, our MALDI-TOF analysis using human heart mitochondrial protein has identified 7 and 8 sequences that could be potentially from ERβ and ERβ3, respectively, but not from ATP synthases. Further, none of the sequences identified by us as those of ERβ and ERβ3 shares m/z targeted by Schwend and Gustafsson in their measurements. Therefore, the claim by Gustafsson's laboratory about false positives in MALDI-TOF detection of ERβ in mitochondria has no relevance to our report.
KW - Estrogen receptor β
KW - False positive
KW - MALDI-TOF
KW - Mitochondria
UR - http://www.scopus.com/inward/record.url?scp=33646830270&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.05.009
DO - 10.1016/j.bbrc.2006.05.009
M3 - Short survey
C2 - 16707101
AN - SCOPUS:33646830270
VL - 345
SP - 917
EP - 918
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -