Abstract
Recently, Schwend and Gustafsson tried to use the MALDI-TOF methods to confirm one of the results reported by Yang et al., which provided definitive evidences to demonstrate the localization of estrogen receptor β (ERβ) in the mitochondria of multiple cell types, using immunocytochemistry, immunoblot, and proteomic approaches. Analysis of the data with the MASCOT database algorithm provided no evidence for the presence of ERβ in the mouse live mitochondria, in which very low ERβ expression has been detected in their own report. On the other hand, our MALDI-TOF analysis using human heart mitochondrial protein has identified 7 and 8 sequences that could be potentially from ERβ and ERβ3, respectively, but not from ATP synthases. Further, none of the sequences identified by us as those of ERβ and ERβ3 shares m/z targeted by Schwend and Gustafsson in their measurements. Therefore, the claim by Gustafsson's laboratory about false positives in MALDI-TOF detection of ERβ in mitochondria has no relevance to our report.
| Original language | English |
|---|---|
| Pages (from-to) | 917-918 |
| Number of pages | 2 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 345 |
| Issue number | 3 |
| DOIs | |
| State | Published - 7 Jul 2006 |
Keywords
- Estrogen receptor β
- False positive
- MALDI-TOF
- Mitochondria