Control of cerebral ischemia with magnetic nanoparticles

Jie Min Jia, Praveen D. Chowdary, Xiaofei Gao, Bo Ci, Wenjun Li, Aditi Mulgaonkar, Erik J. Plautz, Gedaa Hassan, Amit Kumar, Ann M. Stowe, Shao Hua Yang, Wei Zhou, Xiankai Sun, Bianxiao Cui, Woo Ping Ge

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The precise manipulation of microcirculation in mice can facilitate mechanistic studies of brain injury and repair after ischemia, but this manipulation remains a technical challenge, particularly in conscious mice. We developed a technology that uses micromagnets to induce aggregation of magnetic nanoparticles to reversibly occlude blood flow in microvessels. This allowed induction of ischemia in a specific cortical region of conscious mice of any postnatal age, including perinatal and neonatal stages, with precise spatiotemporal control but without surgical intervention of the skull or artery. When combined with longitudinal live-imaging approaches, this technology facilitated the discovery of a feature of the ischemic cascade: selective loss of smooth muscle cells in juveniles but not adults shortly after onset of ischemia and during blood reperfusion.

Original languageEnglish
Pages (from-to)160-166
Number of pages7
JournalNature Methods
Volume14
Issue number2
DOIs
StatePublished - 1 Feb 2017

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    Jia, J. M., Chowdary, P. D., Gao, X., Ci, B., Li, W., Mulgaonkar, A., Plautz, E. J., Hassan, G., Kumar, A., Stowe, A. M., Yang, S. H., Zhou, W., Sun, X., Cui, B., & Ge, W. P. (2017). Control of cerebral ischemia with magnetic nanoparticles. Nature Methods, 14(2), 160-166. https://doi.org/10.1038/nmeth.4105