Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Kate E. Keller, Sanjoy K. Bhattacharya, Theresa Borrás, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark, W. Michael Dismuke, Yiqin Du, Michael H. Elliott, C. Ross Ethier, Jennifer A. Faralli, Thomas F. Freddo, Rudolf Fuchshofer, Michael Giovingo, Haiyan Gong, Pedro Gonzalez, Alex Huang, Murray A. Johnstone, Paul L. Kaufman, Mary J. KelleyPaul A. Knepper, Casey C. Kopczynski, John G. Kuchtey, Rachel W. Kuchtey, Markus H. Kuehn, Raquel L. Lieberman, Shan C. Lin, Paloma Liton, Yutao Liu, Elke Lütjen-Drecoll, Weiming Mao, Marisse Masis-Solano, Fiona McDonnell, Colleen M. McDowell, Darryl R. Overby, Padmanabhan P. Pattabiraman, Vijay K. Raghunathan, P. Vasanth Rao, Douglas J. Rhee, Uttio Roy Chowdhury, Paul Russell, John R. Samples, Donald Schwartz, Evan B. Stubbs, Ernst R. Tamm, James C. Tan, Carol B. Toris, Karen Y. Torrejon, Janice A. Vranka, Mary K. Wirtz, Thomas Yorio, Jie Zhang, Gulab S. Zode, Michael P. Fautsch, Donna M. Peters, Ted S. Acott, W. Daniel Stamer

Research output: Contribution to journalShort survey

28 Scopus citations

Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

Original languageEnglish
Pages (from-to)164-173
Number of pages10
JournalExperimental eye research
Volume171
DOIs
StatePublished - Jun 2018

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Keywords

  • Aqueous humor dynamics
  • Conventional outflow
  • Glaucoma
  • Intraocular pressure
  • Ocular hypertension

Cite this

Keller, K. E., Bhattacharya, S. K., Borrás, T., Brunner, T. M., Chansangpetch, S., Clark, A. F., Dismuke, W. M., Du, Y., Elliott, M. H., Ethier, C. R., Faralli, J. A., Freddo, T. F., Fuchshofer, R., Giovingo, M., Gong, H., Gonzalez, P., Huang, A., Johnstone, M. A., Kaufman, P. L., ... Stamer, W. D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Experimental eye research, 171, 164-173. https://doi.org/10.1016/j.exer.2018.03.001