Confirmation of the identity of human skeletal remains using multiplex PCR amplification and typing kits

M. N. Hochmeister, B. Budowle, U. V. Borer, O. Rudin, M. Bohnert, R. Dirnhofer

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The identity of human skeletal remains found in a wooded area approximately one year after the person was reported missing was provisionally established by routine methods and circumstantial evidence. Multiplex PCR systems-the AmpliType® PM PCR Amplification and Typing Kit and the GenePrint(TM) STR Triplex Amplification and Typing Kit-were used to confirm the identification. DNA profiles from femur bone from the remains were compared with profiles derived from head hairs from a hairbrush recovered in the missing woman's apartment. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. This is the first report of a case using commercially available multiplex PCR amplification and typing kits to confirm the identity of skeletal remains.

Original languageEnglish
Pages (from-to)701-705
Number of pages5
JournalJournal of Forensic Sciences
Volume40
Issue number4
DOIs
StatePublished - 1995

Keywords

  • CSF1PO
  • D7S8
  • GC
  • GYPA
  • HBGG
  • HLA DQA1
  • LDLR
  • TH01
  • TPOX
  • amelogenin
  • deoxyribonucleic acid (DNA)
  • forensic science
  • genetic typing
  • human identification
  • polymerase chain reaction (PCR)
  • sexing
  • short tandem repeats (STR)

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