Abstract
Over 500 population database samples comprising African Americans, Bahamians, and Southwestern Hispanics were typed using the PowerPlex™ 16 and the Profiler Plus™ COfiler™ kits. There was only one sample in which a typing difference was observed. An FGA heterozygote profile was observed using the PowerPlex™ 16 primers, and a single allele FGA profile was observed using Profiler Plus primers. Thus, the extant data suggest that the primers used in the PowerPlex™ 16, Profiler Plus™, and COfiler™ kits are reliable for typing reference samples destined for use in CODIS. In addition, African American, Bahamian, and Southwestern Hispanic databases have been established for the STR loci Penta D and Penta E. Both loci are highly polymorphic. The application of the product rule is valid for estimating the rarity of a multiple loci profile consisting of these two and the 13 core STR loci.
| Original language | English |
|---|---|
| Pages (from-to) | 637-641 |
| Number of pages | 5 |
| Journal | Journal of Forensic Sciences |
| Volume | 46 |
| Issue number | 3 |
| State | Published - 21 Jun 2001 |
Keywords
- Allele drop-out
- AmpFℓSTR® COfiler™ Kit
- AmpFℓSTR® Profiler Plus™ Kit
- CODIS
- Concordance
- Forensic science
- GenePrint® PowerPlex™ 2.1 kit
- Hardy-Weinberg expectations
- Penta D
- Penta E
- PowerPlex™ 16 kit
- Primers
- STR
- Stochastic effects