Concordance study on population database samples using the PowerPlex™ 16 kit and AmpFℓSTR® Profiler Plus™ kit and AmpFℓSTR® COfiler™ kit

B. Budowle, C. J. Sprecher

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Over 500 population database samples comprising African Americans, Bahamians, and Southwestern Hispanics were typed using the PowerPlex™ 16 and the Profiler Plus™ COfiler™ kits. There was only one sample in which a typing difference was observed. An FGA heterozygote profile was observed using the PowerPlex™ 16 primers, and a single allele FGA profile was observed using Profiler Plus primers. Thus, the extant data suggest that the primers used in the PowerPlex™ 16, Profiler Plus™, and COfiler™ kits are reliable for typing reference samples destined for use in CODIS. In addition, African American, Bahamian, and Southwestern Hispanic databases have been established for the STR loci Penta D and Penta E. Both loci are highly polymorphic. The application of the product rule is valid for estimating the rarity of a multiple loci profile consisting of these two and the 13 core STR loci.

Original languageEnglish
Pages (from-to)637-641
Number of pages5
JournalJournal of Forensic Sciences
Volume46
Issue number3
StatePublished - 21 Jun 2001

Keywords

  • Allele drop-out
  • AmpFℓSTR® COfiler™ Kit
  • AmpFℓSTR® Profiler Plus™ Kit
  • CODIS
  • Concordance
  • Forensic science
  • GenePrint® PowerPlex™ 2.1 kit
  • Hardy-Weinberg expectations
  • Penta D
  • Penta E
  • PowerPlex™ 16 kit
  • Primers
  • STR
  • Stochastic effects

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