@inproceedings{a6d116d514184c7280820082a4db31ae,
title = "ConA-based glucose sensing using the long-lifetime azadioxatriangulenium fluorophore",
abstract = "Fluorescent glucose sensing technologies have been identified as possible alternatives to current continuous glucose monitoring approaches. We have recently introduced a new, smart fluorescent ligand to overcome the traditional problems of ConA-based glucose sensors. For this assay to be translated into a continuous glucose monitoring device where both components are free in solution, the molecular weight of the smart fluorescent ligand must be increased. We have identified ovalbumin as a naturally-occurring glycoprotein that could serve as the core-component of a 2nd generation smart fluorescent ligand. It has a single asparagine residue that is capable of displaying an N-linked glycan and a similar isoelectric point to ConA. Thus, binding between ConA and ovalbumin can potentially be monovalent and sugar specific. This work is the preliminary implementation of fluorescently-labeled ovalbumin in the ConA-based assay. We conjugate the red-emitting, long-lifetime azadioxatriangulenium (ADOTA+) dye to ovalbumin, as ADOTA have many advantageous properties to track the equilibrium binding of the assay. The ADOTA-labeled ovalbumin is paired with Alexa Fluor 647-labeled ConA to create a F{\"o}rster Resonance Energy Transfer (FRET) assay that is glucose dependent. The assay responds across the physiologically relevant glucose range (0-500 mg/dL) with increasing intensity from the ADOTA-ovalbumin, showing that the strategy may allow for the translation of the smart fluorescent ligand concept into a continuous glucose monitoring device.",
keywords = "Glucose sensing, competitive binding, energy transfer, fluorescence",
author = "Brian Cummins and Jonathan Simpson and Zygmunt Gryczynski and S{\o}rensen, {Thomas Just} and Laursen, {Bo W.} and Duncan Graham and David Birch and Gerard Cot{\'e}",
year = "2014",
doi = "10.1117/12.2039824",
language = "English",
isbn = "9780819498649",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
booktitle = "Optical Diagnostics and Sensing XIV",
note = "Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics ; Conference date: 03-02-2014 Through 06-02-2014",
}