Composite PAGE: An alternate method for increased separation of amplified short tandem repeat alleles

Miguel Lorente, José A. Lorente, Mark R. Wilson, Bruce Budowle, Enrique Villanueva

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Genetic typing of short tandem repeat (STR) loci may require electrophoretic separation techniques which, depending on the locus, can resolve alleles differing in size by only 2, 3 or 4 base pairs (bps). Many such loci can be separated by horizontal, discontinuous polyacrylamide gel electrophoresis (hd-PAGE). However, some loci present particular separation challenges. A composite (or step) gel system consisting of 2 zones, anodal and cathodal, which differ by pore size and ionic strength, has been developed to augment resolution of DNA fragments without a need for additional electrophoretic equipment. Depending on pore size, as well as ionic strength, initial migration can be increased or decreased to effect resolution. When compared with hd-PAGE, this composite gel electrophoretic system can result in enhanced resolution of PCR amplified STR alleles.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalInternational journal of legal medicine
Volume106
Issue number2
DOIs
StatePublished - 1 Mar 1993

Keywords

  • Composite gels
  • DNA
  • PAGE
  • STR
  • VNTR

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