Composite PAGE: An alternate method for increased separation of amplified short tandem repeat alleles

Miguel Lorente; José A. Lorente; Mark R. Wilson; Bruce Budowle; Enrique Villanueva

doi:10.1007/BF01225043

Composite PAGE: An alternate method for increased separation of amplified short tandem repeat alleles

Miguel Lorente, José A. Lorente, Mark R. Wilson, Bruce Budowle, Enrique Villanueva

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Genetic typing of short tandem repeat (STR) loci may require electrophoretic separation techniques which, depending on the locus, can resolve alleles differing in size by only 2, 3 or 4 base pairs (bps). Many such loci can be separated by horizontal, discontinuous polyacrylamide gel electrophoresis (hd-PAGE). However, some loci present particular separation challenges. A composite (or step) gel system consisting of 2 zones, anodal and cathodal, which differ by pore size and ionic strength, has been developed to augment resolution of DNA fragments without a need for additional electrophoretic equipment. Depending on pore size, as well as ionic strength, initial migration can be increased or decreased to effect resolution. When compared with hd-PAGE, this composite gel electrophoretic system can result in enhanced resolution of PCR amplified STR alleles.

Original language	English
Pages (from-to)	69-73
Number of pages	5
Journal	International journal of legal medicine
Volume	106
Issue number	2
DOIs	https://doi.org/10.1007/BF01225043
State	Published - 1 Mar 1993

Keywords

Composite gels
DNA
PAGE
STR
VNTR

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10.1007/BF01225043

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title = "Composite PAGE: An alternate method for increased separation of amplified short tandem repeat alleles",

abstract = "Genetic typing of short tandem repeat (STR) loci may require electrophoretic separation techniques which, depending on the locus, can resolve alleles differing in size by only 2, 3 or 4 base pairs (bps). Many such loci can be separated by horizontal, discontinuous polyacrylamide gel electrophoresis (hd-PAGE). However, some loci present particular separation challenges. A composite (or step) gel system consisting of 2 zones, anodal and cathodal, which differ by pore size and ionic strength, has been developed to augment resolution of DNA fragments without a need for additional electrophoretic equipment. Depending on pore size, as well as ionic strength, initial migration can be increased or decreased to effect resolution. When compared with hd-PAGE, this composite gel electrophoretic system can result in enhanced resolution of PCR amplified STR alleles.",

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T2 - An alternate method for increased separation of amplified short tandem repeat alleles

AU - Lorente, Miguel

AU - Lorente, José A.

AU - Wilson, Mark R.

AU - Budowle, Bruce

AU - Villanueva, Enrique

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KW - VNTR

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Composite PAGE: An alternate method for increased separation of amplified short tandem repeat alleles

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