Comparison of the performance of different models for the interpretation of low level mixed DNA profiles

Todd W. Bille, Steven M. Weitz, Michael D. Coble, John Buckleton, Jo Anne Bright

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

DNA analyses from forensic casework samples commonly result in complex DNA profiles. Often, these profiles consist of multiple contributors and display multiple stochastic events such as peak height imbalance, allelic or locus drop-out, allelic drop-in, and excessive or indistinguishable stutter. This increased complexity has established a need for more sophisticated methods of DNA mixture interpretation. This study compares the effectiveness of statistical models in the interpretation of artificially created low template two person mixed DNA profiles at varying proportions and template quantities. Two binary models (combined probability of inclusion and random match probability), a semicontinuous (Lab retriever), and continuous model (STRmix™) were compared. Generally, as the sophistication of the models increases, the power of discrimination increases. Differences in discrimination often correlate to each model's ability to use observed data effectively. Binary models require static thresholds resulting in unused data and outliers that may lead to difficult or incorrect interpretation. Semicontinuous and continuous models eliminate the stochastic threshold, however Lab Retriever does not account for stochastic events beyond drop-out and drop-in leading to possible less effective use of the data. STRmix™ incorporates all stochastic events listed above into the calculation making the most effective use of the observed data.

Original languageEnglish
Pages (from-to)3125-3133
Number of pages9
JournalElectrophoresis
Volume35
Issue number21-22
DOIs
StatePublished - 1 Nov 2014

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DNA
Allelic Imbalance
Statistical Models

Keywords

  • Forensic DNA
  • Lab retriever
  • Mixture interpretation
  • RMP
  • STRmix™

Cite this

Bille, Todd W. ; Weitz, Steven M. ; Coble, Michael D. ; Buckleton, John ; Bright, Jo Anne. / Comparison of the performance of different models for the interpretation of low level mixed DNA profiles. In: Electrophoresis. 2014 ; Vol. 35, No. 21-22. pp. 3125-3133.
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Comparison of the performance of different models for the interpretation of low level mixed DNA profiles. / Bille, Todd W.; Weitz, Steven M.; Coble, Michael D.; Buckleton, John; Bright, Jo Anne.

In: Electrophoresis, Vol. 35, No. 21-22, 01.11.2014, p. 3125-3133.

Research output: Contribution to journalArticle

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