Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain

Krishna Midde, Rich Ryan Rich, Marandos Peter Marandos, Fudala Rafal Fudala, Li Amy Li, Gryczynski Ignacy Gryczynski, Julian Borejdo

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Background: Myosin cross-bridges containing phosphorylated and dephosphorylated regulatory light chain may be different. Results: Relaxed cross-bridges, but not active ones, are better ordered in muscle containing dephosphorylated RLC than phosphorylated RLC; they both rotate equally slowly. During contraction phosphorylated cross-bridges rotate faster. Conclusion: Both types of cross-bridges are functionally different. Significance: Phosphorylation of skeletal myosin RLC plays a role in regulation of skeletal muscle contraction.

Original languageEnglish
Pages (from-to)7012-7023
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number10
DOIs
StatePublished - 8 Mar 2013

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