Comparison of ischemia-directed migration of neural precursor cells after intrastriatal, intraventricular, or intravenous transplantation in the rat

Kunlin Jin, Yunjuan Sun, Lin Xie, Xiao Ou Mao, Joycelyn Childs, Alyson Peel, Anna Logvinova, Surita Banwait, David A. Greenberg

Research output: Contribution to journalArticle

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Abstract

Cell replacement therapy may have the potential to promote brain repair and recovery after stroke. To compare how focal cerebral ischemia affects the entry, migration, and phenotypic features of neural precursor cells transplanted by different routes, we administered neuronal precursors from embryonic cerebral cortex of green fluorescent protein (GFP)-expressing transgenic mice to rats that had undergone middle cerebral artery occlusion (MCAO) by the intrastriatal, intraventricular, and intravenous routes. MCAO increased the entry of GFP-immunoreactive cells, most of which expressed neuroepithelial (nestin) or neuronal (doublecortin) markers, from the ventricles and bloodstream into the brain, and enhanced their migration when delivered by any of these routes. Transplanted neural precursors migrated into the ischemic striatum and cerebral cortex. Thus, transplantation of neural precursors by a variety of routes can deliver cells with the potential to replace injured neurons to ischemic brain regions.

Original languageEnglish
Pages (from-to)366-374
Number of pages9
JournalNeurobiology of Disease
Volume18
Issue number2
DOIs
Publication statusPublished - Mar 2005

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Keywords

  • Doublecortin
  • Ischemia
  • Nestin
  • Neural Stem Cells
  • Neurogenesis
  • Stroke
  • Transplantation

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