TY - JOUR
T1 - Comparison of diabetic nephropathy between male and female eNOS-/- db/db mice
AU - Ma, Yuhong
AU - Li, Weizu
AU - Shotorbani, Parisa Yazdizadeh
AU - Dubansky, Brooke Hopkins
AU - Huang, Linjing
AU - Chaudhari, Sarika
AU - Wu, Peiwen
AU - Wang, Lei A.
AU - Ryou, Myoung Gwi
AU - Zhou, Zhengyang
AU - Ma, Rong
N1 - Funding Information:
This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grant 1-RO1-DK-115424-01 (to R. Ma), American Heart Association Southwest Affiliate Grant-In-Aid 16GRNT27780043 (to R. Ma), and an award from the Harry S. Moss Heart Trust (to R. Ma).
Publisher Copyright:
© 2019 the American Physiological Society.
PY - 2019/5
Y1 - 2019/5
N2 - —Sex is an important biological variable that impacts diverse physiological and pathological processes, including the progression of diabetic nephropathy. Diabetic nephropathy is one of the most common complications of diabetes mellitus and is the leading cause of end-stage renal disease. The endothelial nitric oxide synthase-deficient (eNOS+/+) db/db mouse is an appropriate and valuable model to study mechanisms in the development of diabetic nephropathy because of the similarities of the features of diabetic kidney disease in this model to those in humans. The aim of the present study was to determine whether there was a sex difference in renal injury in eNOS+/+ db/db mice. Both male and female eNOS+/+ db/db mice showed hyperglycemia, obesity, and renal hypertrophy. However, there was no significant difference in those variables between male and female mice. Furthermore, both male and female diabetic mice showed progressive albuminuria and significantly greater levels of serum creatinine and blood urea nitrogen compared with the same sex of wild-type mice (nondiabetic controls). Although all three variables in female eNOS+/+ db/db mice had a tendency to be greater than those in male eNOS+/+ db/db mice, those sex differences were not statistically significant. Moreover, both male and female eNOS+/+ db/db mice showed significant mesangial expansion, higher glomerular injury scores, profound renal fibrosis, and substantial accumulation of fibronectin and collagen type IV proteins. However, sex differences in those structural changes were not observed. Similarly, survival rates of male and female eNOS+/+ db/db mice were comparable. Taken together, the results from the present study suggest no sex difference in renal structural and functional damage in eNOS+/+ db/db mice.
AB - —Sex is an important biological variable that impacts diverse physiological and pathological processes, including the progression of diabetic nephropathy. Diabetic nephropathy is one of the most common complications of diabetes mellitus and is the leading cause of end-stage renal disease. The endothelial nitric oxide synthase-deficient (eNOS+/+) db/db mouse is an appropriate and valuable model to study mechanisms in the development of diabetic nephropathy because of the similarities of the features of diabetic kidney disease in this model to those in humans. The aim of the present study was to determine whether there was a sex difference in renal injury in eNOS+/+ db/db mice. Both male and female eNOS+/+ db/db mice showed hyperglycemia, obesity, and renal hypertrophy. However, there was no significant difference in those variables between male and female mice. Furthermore, both male and female diabetic mice showed progressive albuminuria and significantly greater levels of serum creatinine and blood urea nitrogen compared with the same sex of wild-type mice (nondiabetic controls). Although all three variables in female eNOS+/+ db/db mice had a tendency to be greater than those in male eNOS+/+ db/db mice, those sex differences were not statistically significant. Moreover, both male and female eNOS+/+ db/db mice showed significant mesangial expansion, higher glomerular injury scores, profound renal fibrosis, and substantial accumulation of fibronectin and collagen type IV proteins. However, sex differences in those structural changes were not observed. Similarly, survival rates of male and female eNOS+/+ db/db mice were comparable. Taken together, the results from the present study suggest no sex difference in renal structural and functional damage in eNOS+/+ db/db mice.
KW - Diabetic nephropathy
KW - Endothelial nitric oxide synthase-deficient db/db mouse
KW - Mouse model of diabetic nephropathy
KW - Renal injury
KW - Sex difference
UR - http://www.scopus.com/inward/record.url?scp=85065417109&partnerID=8YFLogxK
U2 - 10.1152/ajprenal.00023.2019
DO - 10.1152/ajprenal.00023.2019
M3 - Article
C2 - 30810354
AN - SCOPUS:85065417109
SN - 0363-6127
VL - 316
SP - F889-F897
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 5
ER -