Comparison of diabetic nephropathy between male and female eNOS -/- db/db mice

Yuhong Ma, Weizu Li, Parisa Yazdizadeh Shotorbani, Brooke Hopkins Dubansky, Linjing Huang, Sarika Chaudhari, Peiwen Wu, Lei A. Wang, Myoung Gwi Ryou, Zhengyang Zhou, Rong Ma

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Sex is an important biological variable that impacts diverse physiological and pathological processes, including the progression of diabetic nephropathy. Diabetic nephropathy is one of the most common complications of diabetes mellitus and is the leading cause of end-stage renal disease. The endothelial nitric oxide synthase-deficient (eNOS-/-) db/db mouse is an appropriate and valuable model to study mechanisms in the development of diabetic nephropathy because of the similarities of the features of diabetic kidney disease in this model to those in humans. The aim of the present study was to determine whether there was a sex difference in renal injury in eNOS-/-db/db mice. Both male and female eNOS-/-db/db mice showed hyperglycemia, obesity, and renal hypertrophy. However, there was no significant difference in those variables between male and female mice. Furthermore, both male and female diabetic mice showed progressive albuminuria and significantly greater levels of serum creatinine and blood urea nitrogen compared with the same sex of wild-type mice (nondiabetic controls). Although all three variables in female eNOS-/-db/db mice had a tendency to be greater than those in male eNOS-/-db/db mice, those sex differences were not statistically significant. Moreover, both male and female eNOS-/-db/db mice showed significant mesangial expansion, higher glomerular injury scores, profound renal fibrosis, and substantial accumulation of fibronectin and collagen type IV proteins. However, sex differences in those structural changes were not observed. Similarly, survival rates of male and female eNOS-/-db/db mice were comparable. Taken together, the results from the present study suggest no sex difference in renal structural and functional damage in eNOS-/-db/db mice.

Original languageEnglish
Pages (from-to)F889-F897
JournalAmerican journal of physiology. Renal physiology
Volume316
Issue number5
DOIs
StatePublished - 1 May 2019

Fingerprint

Diabetic Nephropathies
Nitric Oxide Synthase Type III
Sex Characteristics
Kidney
Physiological Phenomena
Albuminuria
Collagen Type IV
Blood Urea Nitrogen
Wounds and Injuries
Pathologic Processes
Diabetes Complications
Fibronectins
Hyperglycemia
Hypertrophy
Chronic Kidney Failure
Creatinine
Fibrosis
Obesity

Keywords

  • diabetic nephropathy
  • endothelial nitric oxide synthase-deficient / mouse
  • mouse model of diabetic nephropathy
  • renal injury
  • sex difference

Cite this

Ma, Yuhong ; Li, Weizu ; Yazdizadeh Shotorbani, Parisa ; Dubansky, Brooke Hopkins ; Huang, Linjing ; Chaudhari, Sarika ; Wu, Peiwen ; Wang, Lei A. ; Ryou, Myoung Gwi ; Zhou, Zhengyang ; Ma, Rong. / Comparison of diabetic nephropathy between male and female eNOS -/- db/db mice In: American journal of physiology. Renal physiology. 2019 ; Vol. 316, No. 5. pp. F889-F897.
@article{bcec5ab115764d52b78a101090a8ac58,
title = "Comparison of diabetic nephropathy between male and female eNOS -/- db/db mice",
abstract = "Sex is an important biological variable that impacts diverse physiological and pathological processes, including the progression of diabetic nephropathy. Diabetic nephropathy is one of the most common complications of diabetes mellitus and is the leading cause of end-stage renal disease. The endothelial nitric oxide synthase-deficient (eNOS-/-) db/db mouse is an appropriate and valuable model to study mechanisms in the development of diabetic nephropathy because of the similarities of the features of diabetic kidney disease in this model to those in humans. The aim of the present study was to determine whether there was a sex difference in renal injury in eNOS-/-db/db mice. Both male and female eNOS-/-db/db mice showed hyperglycemia, obesity, and renal hypertrophy. However, there was no significant difference in those variables between male and female mice. Furthermore, both male and female diabetic mice showed progressive albuminuria and significantly greater levels of serum creatinine and blood urea nitrogen compared with the same sex of wild-type mice (nondiabetic controls). Although all three variables in female eNOS-/-db/db mice had a tendency to be greater than those in male eNOS-/-db/db mice, those sex differences were not statistically significant. Moreover, both male and female eNOS-/-db/db mice showed significant mesangial expansion, higher glomerular injury scores, profound renal fibrosis, and substantial accumulation of fibronectin and collagen type IV proteins. However, sex differences in those structural changes were not observed. Similarly, survival rates of male and female eNOS-/-db/db mice were comparable. Taken together, the results from the present study suggest no sex difference in renal structural and functional damage in eNOS-/-db/db mice.",
keywords = "diabetic nephropathy, endothelial nitric oxide synthase-deficient / mouse, mouse model of diabetic nephropathy, renal injury, sex difference",
author = "Yuhong Ma and Weizu Li and {Yazdizadeh Shotorbani}, Parisa and Dubansky, {Brooke Hopkins} and Linjing Huang and Sarika Chaudhari and Peiwen Wu and Wang, {Lei A.} and Ryou, {Myoung Gwi} and Zhengyang Zhou and Rong Ma",
year = "2019",
month = "5",
day = "1",
doi = "10.1152/ajprenal.00023.2019",
language = "English",
volume = "316",
pages = "F889--F897",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "5",

}

Ma, Y, Li, W, Yazdizadeh Shotorbani, P, Dubansky, BH, Huang, L, Chaudhari, S, Wu, P, Wang, LA, Ryou, MG, Zhou, Z & Ma, R 2019, ' Comparison of diabetic nephropathy between male and female eNOS -/- db/db mice ' American journal of physiology. Renal physiology, vol. 316, no. 5, pp. F889-F897. https://doi.org/10.1152/ajprenal.00023.2019

Comparison of diabetic nephropathy between male and female eNOS -/- db/db mice . / Ma, Yuhong; Li, Weizu; Yazdizadeh Shotorbani, Parisa; Dubansky, Brooke Hopkins; Huang, Linjing; Chaudhari, Sarika; Wu, Peiwen; Wang, Lei A.; Ryou, Myoung Gwi; Zhou, Zhengyang; Ma, Rong.

In: American journal of physiology. Renal physiology, Vol. 316, No. 5, 01.05.2019, p. F889-F897.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Comparison of diabetic nephropathy between male and female eNOS -/- db/db mice

AU - Ma, Yuhong

AU - Li, Weizu

AU - Yazdizadeh Shotorbani, Parisa

AU - Dubansky, Brooke Hopkins

AU - Huang, Linjing

AU - Chaudhari, Sarika

AU - Wu, Peiwen

AU - Wang, Lei A.

AU - Ryou, Myoung Gwi

AU - Zhou, Zhengyang

AU - Ma, Rong

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Sex is an important biological variable that impacts diverse physiological and pathological processes, including the progression of diabetic nephropathy. Diabetic nephropathy is one of the most common complications of diabetes mellitus and is the leading cause of end-stage renal disease. The endothelial nitric oxide synthase-deficient (eNOS-/-) db/db mouse is an appropriate and valuable model to study mechanisms in the development of diabetic nephropathy because of the similarities of the features of diabetic kidney disease in this model to those in humans. The aim of the present study was to determine whether there was a sex difference in renal injury in eNOS-/-db/db mice. Both male and female eNOS-/-db/db mice showed hyperglycemia, obesity, and renal hypertrophy. However, there was no significant difference in those variables between male and female mice. Furthermore, both male and female diabetic mice showed progressive albuminuria and significantly greater levels of serum creatinine and blood urea nitrogen compared with the same sex of wild-type mice (nondiabetic controls). Although all three variables in female eNOS-/-db/db mice had a tendency to be greater than those in male eNOS-/-db/db mice, those sex differences were not statistically significant. Moreover, both male and female eNOS-/-db/db mice showed significant mesangial expansion, higher glomerular injury scores, profound renal fibrosis, and substantial accumulation of fibronectin and collagen type IV proteins. However, sex differences in those structural changes were not observed. Similarly, survival rates of male and female eNOS-/-db/db mice were comparable. Taken together, the results from the present study suggest no sex difference in renal structural and functional damage in eNOS-/-db/db mice.

AB - Sex is an important biological variable that impacts diverse physiological and pathological processes, including the progression of diabetic nephropathy. Diabetic nephropathy is one of the most common complications of diabetes mellitus and is the leading cause of end-stage renal disease. The endothelial nitric oxide synthase-deficient (eNOS-/-) db/db mouse is an appropriate and valuable model to study mechanisms in the development of diabetic nephropathy because of the similarities of the features of diabetic kidney disease in this model to those in humans. The aim of the present study was to determine whether there was a sex difference in renal injury in eNOS-/-db/db mice. Both male and female eNOS-/-db/db mice showed hyperglycemia, obesity, and renal hypertrophy. However, there was no significant difference in those variables between male and female mice. Furthermore, both male and female diabetic mice showed progressive albuminuria and significantly greater levels of serum creatinine and blood urea nitrogen compared with the same sex of wild-type mice (nondiabetic controls). Although all three variables in female eNOS-/-db/db mice had a tendency to be greater than those in male eNOS-/-db/db mice, those sex differences were not statistically significant. Moreover, both male and female eNOS-/-db/db mice showed significant mesangial expansion, higher glomerular injury scores, profound renal fibrosis, and substantial accumulation of fibronectin and collagen type IV proteins. However, sex differences in those structural changes were not observed. Similarly, survival rates of male and female eNOS-/-db/db mice were comparable. Taken together, the results from the present study suggest no sex difference in renal structural and functional damage in eNOS-/-db/db mice.

KW - diabetic nephropathy

KW - endothelial nitric oxide synthase-deficient / mouse

KW - mouse model of diabetic nephropathy

KW - renal injury

KW - sex difference

UR - http://www.scopus.com/inward/record.url?scp=85065417109&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00023.2019

DO - 10.1152/ajprenal.00023.2019

M3 - Article

VL - 316

SP - F889-F897

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 5

ER -