TY - JOUR
T1 - Colonizing populations of Candida albicans are clonal in origin but undergo microevolution through C1 fragment reorganization as demonstrated by DNA fingerprinting and C1 sequencing
AU - Lockhart, S. R.
AU - Fritch, J. J.
AU - Meier, A. S.
AU - Schroppel, K.
AU - Srikantha, T.
AU - Galask, R.
AU - Soll, D. R.
PY - 1995
Y1 - 1995
N2 - The genetic homogeneity of nine commensal and infecting populations of Candida albicans has been assessed by fingerprinting multiple isolates from each population by Southern blot hybridization first with the Ca3 probe and then with the 0.98-kb C1 fragment of the Ca3 probe. The isolates from each population were highly related, demonstrating the clonal origin of each population, but each population contained minor variants, demonstrating microevolution. Variation in each case was limited to bands of the Ca3 fingerprint pattern which hybridized with the 0.98-kb C1 fragment. The C1 fragment was therefore sequenced and demonstrated to contain an RPS repetitive element. The C1 fragment also contained part or all of a true end of the RPS element. These results, therefore, demonstrate that most colonizing C. albicans populations in nonimmunosuppressed patients are clonal, that microevolution can be detected in every colonizing population by C1 hybridization, and that C1 contains the repeat RPS element.
AB - The genetic homogeneity of nine commensal and infecting populations of Candida albicans has been assessed by fingerprinting multiple isolates from each population by Southern blot hybridization first with the Ca3 probe and then with the 0.98-kb C1 fragment of the Ca3 probe. The isolates from each population were highly related, demonstrating the clonal origin of each population, but each population contained minor variants, demonstrating microevolution. Variation in each case was limited to bands of the Ca3 fingerprint pattern which hybridized with the 0.98-kb C1 fragment. The C1 fragment was therefore sequenced and demonstrated to contain an RPS repetitive element. The C1 fragment also contained part or all of a true end of the RPS element. These results, therefore, demonstrate that most colonizing C. albicans populations in nonimmunosuppressed patients are clonal, that microevolution can be detected in every colonizing population by C1 hybridization, and that C1 contains the repeat RPS element.
UR - http://www.scopus.com/inward/record.url?scp=0028997579&partnerID=8YFLogxK
U2 - 10.1128/jcm.33.6.1501-1509.1995
DO - 10.1128/jcm.33.6.1501-1509.1995
M3 - Article
C2 - 7650175
AN - SCOPUS:0028997579
SN - 0095-1137
VL - 33
SP - 1501
EP - 1509
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 6
ER -