Co-expression of the 5-HT3B subunit with the 5-HT3A receptor reduces alcohol sensitivity

Volodya Hayrapetyan; Monica Jenschke; Glenn H. Dillon; Tina K. Machu

doi:10.1016/j.molbrainres.2005.09.011

Co-expression of the 5-HT_3B subunit with the 5-HT_3A receptor reduces alcohol sensitivity

Volodya Hayrapetyan, Monica Jenschke, Glenn H. Dillon, Tina K. Machu

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Allosteric modulation of mouse 5-Hydroxytryptamine_3A (5-HT _3A) and 5-HT_3A/B receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT_3A receptor function, but had no effect on mouse 5-HT_3A/B receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT _3A/B receptor function was much less than that observed in the mouse 5-HT_3A receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20-80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT₃ receptors.

Original language	English
Pages (from-to)	146-150
Number of pages	5
Journal	Molecular Brain Research
Volume	142
Issue number	2
DOIs	https://doi.org/10.1016/j.molbrainres.2005.09.011
State	Published - 14 Dec 2005

Keywords

5-HT
5-HT
Ethanol
Ligand-gated ion channel
Trichloroethanol

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10.1016/j.molbrainres.2005.09.011

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title = "Co-expression of the 5-HT3B subunit with the 5-HT3A receptor reduces alcohol sensitivity",

abstract = "Allosteric modulation of mouse 5-Hydroxytryptamine3A (5-HT 3A) and 5-HT3A/B receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT3A receptor function, but had no effect on mouse 5-HT3A/B receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT 3A/B receptor function was much less than that observed in the mouse 5-HT3A receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20-80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT3 receptors.",

keywords = "5-HT, 5-HT, Ethanol, Ligand-gated ion channel, Trichloroethanol",

author = "Volodya Hayrapetyan and Monica Jenschke and Dillon, {Glenn H.} and Machu, {Tina K.}",

note = "Funding Information: This work was supported by the National Institutes of Health, AA12643 (TKM).",

year = "2005",

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doi = "10.1016/j.molbrainres.2005.09.011",

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AU - Hayrapetyan, Volodya

AU - Jenschke, Monica

AU - Dillon, Glenn H.

AU - Machu, Tina K.

N1 - Funding Information: This work was supported by the National Institutes of Health, AA12643 (TKM).

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AB - Allosteric modulation of mouse 5-Hydroxytryptamine3A (5-HT 3A) and 5-HT3A/B receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT3A receptor function, but had no effect on mouse 5-HT3A/B receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT 3A/B receptor function was much less than that observed in the mouse 5-HT3A receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20-80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT3 receptors.

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KW - Ethanol

KW - Ligand-gated ion channel

KW - Trichloroethanol

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JO - Molecular Brain Research

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ER -

Co-expression of the 5-HT_3B subunit with the 5-HT_3A receptor reduces alcohol sensitivity

Abstract

Keywords

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