TY - JOUR
T1 - Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25
AU - Grossman, Alex
AU - Mittrücker, Hans Willi
AU - Nicholl, Jillian
AU - Suzuki, Akira
AU - Chung, Stephen
AU - Antonio, Laarni
AU - Suggs, Sid
AU - Sutherland, Grant R.
AU - Siderovski, David P.
AU - Mak, Tak W.
N1 - Funding Information:
We thank H. Messner for providing cell lines, C. Richardson for critical reading of the manuscript, and S. Yoshinaga, A. Hessel, and F. Kiefer for helpful comments. H.-W. MittruÈ cker is supported by a grant from the Deutsche Forschungsgemeinschaft.
PY - 1996/10/15
Y1 - 1996/10/15
N2 - The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of ~5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU.1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25.
AB - The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of ~5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU.1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25.
UR - http://www.scopus.com/inward/record.url?scp=0030588140&partnerID=8YFLogxK
U2 - 10.1006/geno.1996.0547
DO - 10.1006/geno.1996.0547
M3 - Article
C2 - 8921401
AN - SCOPUS:0030588140
SN - 0888-7543
VL - 37
SP - 229
EP - 233
JO - Genomics
JF - Genomics
IS - 2
ER -