Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Brian W. Biegler; Shirley X. Yan; Sterling B. Ortega; Deepani K. Tennakoon; Michael K. Racke; Nitin J. Karandikar

doi:10.1016/j.jneuroim.2011.02.001

Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Brian W. Biegler, Shirley X. Yan, Sterling B. Ortega, Deepani K. Tennakoon, Michael K. Racke, Nitin J. Karandikar

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.

Original language	English
Pages (from-to)	131-140
Number of pages	10
Journal	Journal of Neuroimmunology
Volume	234
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2011.02.001
State	Published - May 2011

Keywords

CD4
CD8
CDR3
Multiple sclerosis
Myelin
TCR

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10.1016/j.jneuroim.2011.02.001

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title = "Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis",

abstract = "Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.",

keywords = "CD4, CD8, CDR3, Multiple sclerosis, Myelin, TCR",

author = "Biegler, {Brian W.} and Yan, {Shirley X.} and Ortega, {Sterling B.} and Tennakoon, {Deepani K.} and Racke, {Michael K.} and Karandikar, {Nitin J.}",

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AU - Biegler, Brian W.

AU - Yan, Shirley X.

AU - Ortega, Sterling B.

AU - Tennakoon, Deepani K.

AU - Racke, Michael K.

AU - Karandikar, Nitin J.

PY - 2011/5

Y1 - 2011/5

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AB - Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.

KW - CD4

KW - CD8

KW - CDR3

KW - Multiple sclerosis

KW - Myelin

KW - TCR

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Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Abstract

Keywords

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