Abstract
Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.
Original language | English |
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Pages (from-to) | 131-140 |
Number of pages | 10 |
Journal | Journal of Neuroimmunology |
Volume | 234 |
Issue number | 1-2 |
DOIs | |
State | Published - May 2011 |
Keywords
- CD4
- CD8
- CDR3
- Multiple sclerosis
- Myelin
- TCR