TY - JOUR
T1 - Cigarette smoke exposure alters [14c]arachidonic acid metabolism in aortas and platelets of rats fed various levels of selenium and vitamin e
AU - Valentovic, M. A.
AU - Gairola, C.
AU - Lubawy, W. C.
N1 - Funding Information:
This work was supported in part by University of Kentucky Tobacco and Health Research Institute Projects 4B006 and 4A015. The authors are indebted to P. Wiseman for smoke inhalation, B. Brown and J. Atkinson for technical assistance, and the Scientific Information Section of the Tobacco and Health Research Institute for their excellent editorial, literature, and graphic services. Requests for reprints should be sent to W. C. Lubawy, College of Pharmacy, University of Kentucky, Lexington, Kentucky 40506.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - Tats were placed on a basal diet supplemented with 0,0.03, or 3 ppm selenium and 0 or 20 ppm vitamin E (or 41-43 wk. Selenium deficiency decreased hepatic glutathione peroxidase activity and lowered both aortic prostacyclin (PGI2) and platelet thromboxane (TXA2) production compared to selenium- and vitamin E-supplemented animals. Vitamin E deficiency increased hepatic lipid peroxidation and decreased aortic PGI2synthesis. Rats exposed daily for 31-32 wk to fresh smoke from a UK 2R1 reference cigarette had carboxyhemoglobin levels of 0.75 + 0.12 and 4.73 ± 0.12% in sham- and smoke-exposed groups, respectively. Animals chronically exposed to cigarette smoke displayed a nearly twofold increase in pulmonary aryl hydrocarbon hydroxylase activity. Smoke exposure produced a 26-33% decrease in aortic PCI, synthesis compared to shams in the Se2E20, Se0 03E20, and Se3E0groups. Smoking also increased platelet thromboxane 91% and 98%. in the Se003E10and S3E0groups compared to shams. It is concluded that cigarette-smoke exposure and selenium or vitamin E deficiency alter aortic PGI, and platelet TXA1production.
AB - Tats were placed on a basal diet supplemented with 0,0.03, or 3 ppm selenium and 0 or 20 ppm vitamin E (or 41-43 wk. Selenium deficiency decreased hepatic glutathione peroxidase activity and lowered both aortic prostacyclin (PGI2) and platelet thromboxane (TXA2) production compared to selenium- and vitamin E-supplemented animals. Vitamin E deficiency increased hepatic lipid peroxidation and decreased aortic PGI2synthesis. Rats exposed daily for 31-32 wk to fresh smoke from a UK 2R1 reference cigarette had carboxyhemoglobin levels of 0.75 + 0.12 and 4.73 ± 0.12% in sham- and smoke-exposed groups, respectively. Animals chronically exposed to cigarette smoke displayed a nearly twofold increase in pulmonary aryl hydrocarbon hydroxylase activity. Smoke exposure produced a 26-33% decrease in aortic PCI, synthesis compared to shams in the Se2E20, Se0 03E20, and Se3E0groups. Smoking also increased platelet thromboxane 91% and 98%. in the Se003E10and S3E0groups compared to shams. It is concluded that cigarette-smoke exposure and selenium or vitamin E deficiency alter aortic PGI, and platelet TXA1production.
UR - http://www.scopus.com/inward/record.url?scp=0022344429&partnerID=8YFLogxK
U2 - 10.1080/15287398509530676
DO - 10.1080/15287398509530676
M3 - Article
C2 - 3928899
AN - SCOPUS:0022344429
SN - 0098-4108
VL - 15
SP - 493
EP - 502
JO - Journal of Toxicology and Environmental Health
JF - Journal of Toxicology and Environmental Health
IS - 3-4
ER -