Chronic cigarette smoke exposure adversely alters 14C-arachidonic acid metabolism in rat lungs, aortas and platelets

William C. Lubawy; Monica A. Valentovic; John E. Atkinson; Gary C. Gairola

doi:10.1016/0024-3205(83)90133-9

Chronic cigarette smoke exposure adversely alters ¹⁴C-arachidonic acid metabolism in rat lungs, aortas and platelets

William C. Lubawy, Monica A. Valentovic, John E. Atkinson, Gary C. Gairola

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Male rats were exposed to freshly generated cigarette smoke once daily, 5 times a week for 10 weeks. Inhalation of smoke was verified by elevated carboxyhemoglobin in blood sampled immediately after smoke exposure and by increased lung aryl hydrocarbon hydroxylase activity 24 hours after the last smoke exposure. Aortic rings isolated from smoke-exposed rats synthesized less prostacyclin (PGI₂) from ¹⁴C-arachidonic acid than rings from sham rats. Platelets from smoke-exposed rats synthesized more thromboxane (TXA₂) from ¹⁴C-arachidonic acid than platelets from room controls but not those from sham rats. Lung microsomes from smoke-exposed rats synthesized more TXA₂ and had a lower PGI₂/TXA₂ ratio than lung microsomes from room controls and shams. It is concluded that chronic cigarette smoke exposure alters arachidonic acid metabolism in aortas, platelets and lungs in a manner resulting in decreased PGI₂ and increased TXA₂, thereby creating a condition favoring platelet aggregation and a variety of cardiovascular diseases.

Original language	English
Pages (from-to)	577-584
Number of pages	8
Journal	Life Sciences
Volume	33
Issue number	6
DOIs	https://doi.org/10.1016/0024-3205(83)90133-9
State	Published - 8 Aug 1983

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title = "Chronic cigarette smoke exposure adversely alters 14C-arachidonic acid metabolism in rat lungs, aortas and platelets",

abstract = "Male rats were exposed to freshly generated cigarette smoke once daily, 5 times a week for 10 weeks. Inhalation of smoke was verified by elevated carboxyhemoglobin in blood sampled immediately after smoke exposure and by increased lung aryl hydrocarbon hydroxylase activity 24 hours after the last smoke exposure. Aortic rings isolated from smoke-exposed rats synthesized less prostacyclin (PGI2) from 14C-arachidonic acid than rings from sham rats. Platelets from smoke-exposed rats synthesized more thromboxane (TXA2) from 14C-arachidonic acid than platelets from room controls but not those from sham rats. Lung microsomes from smoke-exposed rats synthesized more TXA2 and had a lower PGI2/TXA2 ratio than lung microsomes from room controls and shams. It is concluded that chronic cigarette smoke exposure alters arachidonic acid metabolism in aortas, platelets and lungs in a manner resulting in decreased PGI2 and increased TXA2, thereby creating a condition favoring platelet aggregation and a variety of cardiovascular diseases.",

author = "Lubawy, {William C.} and Valentovic, {Monica A.} and Atkinson, {John E.} and Gairola, {Gary C.}",

note = "Funding Information: This project was supported in part by KTRB 4F006 and KTRB 4A015 from the Kentucky Tobacco Research Board. The authors thank B. Culpepper and J. Glass for excellent technical assistance, P. Wiseman for smoke inhalation, and the Scientific Information Section of the Tobacco and Health Research Institute for excellent editorial and literature services.",

year = "1983",

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doi = "10.1016/0024-3205(83)90133-9",

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AU - Lubawy, William C.

AU - Valentovic, Monica A.

AU - Atkinson, John E.

AU - Gairola, Gary C.

N1 - Funding Information: This project was supported in part by KTRB 4F006 and KTRB 4A015 from the Kentucky Tobacco Research Board. The authors thank B. Culpepper and J. Glass for excellent technical assistance, P. Wiseman for smoke inhalation, and the Scientific Information Section of the Tobacco and Health Research Institute for excellent editorial and literature services.

PY - 1983/8/8

Y1 - 1983/8/8

N2 - Male rats were exposed to freshly generated cigarette smoke once daily, 5 times a week for 10 weeks. Inhalation of smoke was verified by elevated carboxyhemoglobin in blood sampled immediately after smoke exposure and by increased lung aryl hydrocarbon hydroxylase activity 24 hours after the last smoke exposure. Aortic rings isolated from smoke-exposed rats synthesized less prostacyclin (PGI2) from 14C-arachidonic acid than rings from sham rats. Platelets from smoke-exposed rats synthesized more thromboxane (TXA2) from 14C-arachidonic acid than platelets from room controls but not those from sham rats. Lung microsomes from smoke-exposed rats synthesized more TXA2 and had a lower PGI2/TXA2 ratio than lung microsomes from room controls and shams. It is concluded that chronic cigarette smoke exposure alters arachidonic acid metabolism in aortas, platelets and lungs in a manner resulting in decreased PGI2 and increased TXA2, thereby creating a condition favoring platelet aggregation and a variety of cardiovascular diseases.

AB - Male rats were exposed to freshly generated cigarette smoke once daily, 5 times a week for 10 weeks. Inhalation of smoke was verified by elevated carboxyhemoglobin in blood sampled immediately after smoke exposure and by increased lung aryl hydrocarbon hydroxylase activity 24 hours after the last smoke exposure. Aortic rings isolated from smoke-exposed rats synthesized less prostacyclin (PGI2) from 14C-arachidonic acid than rings from sham rats. Platelets from smoke-exposed rats synthesized more thromboxane (TXA2) from 14C-arachidonic acid than platelets from room controls but not those from sham rats. Lung microsomes from smoke-exposed rats synthesized more TXA2 and had a lower PGI2/TXA2 ratio than lung microsomes from room controls and shams. It is concluded that chronic cigarette smoke exposure alters arachidonic acid metabolism in aortas, platelets and lungs in a manner resulting in decreased PGI2 and increased TXA2, thereby creating a condition favoring platelet aggregation and a variety of cardiovascular diseases.

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EP - 584

JO - Life Sciences

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ER -

Chronic cigarette smoke exposure adversely alters ¹⁴C-arachidonic acid metabolism in rat lungs, aortas and platelets

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