TY - JOUR
T1 - Characterization of p-bis(O-methylstyryl) benzene as a lifetime and anisotropy decay standard for two-photon induced fluorescence
AU - Lakowicz, Joseph R.
AU - Gryczynski, Ignacy
N1 - Funding Information:
The authors acknowledges upport from the NationalS cienceF oundation( DMB-8804931t)h, e National Institutes of Health (RR-04800, RR-07510a nd RR-08119)a nd the Medical Biotechnology Center and GraduateS chool at the Uni-versityo f Maryland.
PY - 1993/7
Y1 - 1993/7
N2 - We describe the fluorescence spectral properties of p-bis(O-methylstyryl)benzene (bis-MSB) as a standard for time-resolved measurements of two-photon induced fluorescence. Bis-MSB displays the same single exponential intensity decay in several solvents for one- and two-photon excitation. The anisotropy decay displays the same single correlation time of one- and two-photon excitation. The amplitudes of the anisotropy decay are distinct for one- and two-photon excitation. At some excitation wavelengths the anisotropy amplitude appears to be solely the result of one- and two-photon photoselection, but at shorter wavelengths the anisotropy amplitudes are not related by single constant factor. The absorption range of bis-MSB makes it a suitable standard for one- and two-photon excitation of intrinsic protein fluorescence and extrinsic fluorophores.
AB - We describe the fluorescence spectral properties of p-bis(O-methylstyryl)benzene (bis-MSB) as a standard for time-resolved measurements of two-photon induced fluorescence. Bis-MSB displays the same single exponential intensity decay in several solvents for one- and two-photon excitation. The anisotropy decay displays the same single correlation time of one- and two-photon excitation. The amplitudes of the anisotropy decay are distinct for one- and two-photon excitation. At some excitation wavelengths the anisotropy amplitude appears to be solely the result of one- and two-photon photoselection, but at shorter wavelengths the anisotropy amplitudes are not related by single constant factor. The absorption range of bis-MSB makes it a suitable standard for one- and two-photon excitation of intrinsic protein fluorescence and extrinsic fluorophores.
KW - Fluorescence intensity decay
KW - Protein fluorescence
KW - Two-photon induced fluorescence
KW - p-Bis(O-methylstyryl)benzene
UR - http://www.scopus.com/inward/record.url?scp=0027291681&partnerID=8YFLogxK
U2 - 10.1016/0301-4622(93)80027-G
DO - 10.1016/0301-4622(93)80027-G
M3 - Article
C2 - 8364146
AN - SCOPUS:0027291681
VL - 47
SP - 1
EP - 7
JO - Biophysical Chemistry
JF - Biophysical Chemistry
SN - 0301-4622
IS - 1
ER -