@article{f5119fee841548f99d06dcc0e0be5b47,
title = "Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment",
abstract = "Using a plasmid DNA model and total genomic DNA (gDNA) reference samples, we examined the performance and forensic utility of a quantitative allele-discriminatory real-time PCR assay relying on TaqMan probes targeting the mitochondrial single nucleotide polymorphism (mtSNP) 16519T/C. For allele-calling and allele proportion assessment we used absolute quantification of both alleles as well as relative quantification based on endpoint fluorescence.",
keywords = "Allele proportion assessment, Allele-discriminatory rtPCR, Forensic science",
author = "H. Niederst{\"a}tter and Coble, {M. D.} and Parsons, {T. J.} and W. Parson",
note = "Funding Information: Part of this work was supported by the National Institute of Justice grant 2000-1J-CX-K010 to T.J.P. The opinions and assertions contained herein are solely those of the authors and are not to be construed as official or as views of the U.S. Department of Defense, the U.S. Department of the Army, or the U.S. Department of Justice.",
year = "2006",
month = apr,
doi = "10.1016/j.ics.2005.09.021",
language = "English",
volume = "1288",
pages = "1--3",
journal = "International Congress Series",
issn = "0531-5131",
publisher = "Elsevier BV",
}