Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment

H. Niederstätter, Michael Dewitt Coble, T. J. Parsons, W. Parson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Using a plasmid DNA model and total genomic DNA (gDNA) reference samples, we examined the performance and forensic utility of a quantitative allele-discriminatory real-time PCR assay relying on TaqMan probes targeting the mitochondrial single nucleotide polymorphism (mtSNP) 16519T/C. For allele-calling and allele proportion assessment we used absolute quantification of both alleles as well as relative quantification based on endpoint fluorescence.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalInternational Congress Series
Volume1288
DOIs
StatePublished - 1 Apr 2006

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Mitochondrial DNA
Single Nucleotide Polymorphism
Real-Time Polymerase Chain Reaction
Alleles
DNA
Plasmids
Fluorescence

Keywords

  • Allele proportion assessment
  • Allele-discriminatory rtPCR
  • Forensic science

Cite this

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Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment. / Niederstätter, H.; Coble, Michael Dewitt; Parsons, T. J.; Parson, W.

In: International Congress Series, Vol. 1288, 01.04.2006, p. 1-3.

Research output: Contribution to journalArticle

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