TY - JOUR
T1 - Characterization of lecithin:cholesterol acyltransferase from human plasma
T2 - Purification of the enzyme
AU - Chong, Kui Song
AU - Davidson, Leo
AU - Huttash, R. G.
AU - Lacko, Andras G.
N1 - Funding Information:
The work reported in this communication was supported by grants from the Robert A. Welch Foundation (B-725), the National Institutes of Health (AG-01450), and the Faculty Research Fund of the Texas College of Osteopathic Medicine. The authors thank Mr. W. C. Ruckle for technical assistance.
PY - 1981/10/1
Y1 - 1981/10/1
N2 - Lecithin:cholesterol acyltransferase was isolated from a human plasma fraction in a homogeneous state by an efficient and highly reproducible procedure. The purification scheme involved: (1) hydrophobic affinity chromatography, (2) DEAE-agarose chromatography, (3) delipidation, (4) a second DEAE-agarose chromatography, and (5) a combination of hydroxylapatite and antibody adsorption chromatography. The final product was purified 20,000-fold over the starting material and was found to be free of any polypeptide contaminant as indicated by gel electrophoresis, sedimentation equilibrium ultracentrifugation, immunodiffusion, and immunoelectrophoresis.
AB - Lecithin:cholesterol acyltransferase was isolated from a human plasma fraction in a homogeneous state by an efficient and highly reproducible procedure. The purification scheme involved: (1) hydrophobic affinity chromatography, (2) DEAE-agarose chromatography, (3) delipidation, (4) a second DEAE-agarose chromatography, and (5) a combination of hydroxylapatite and antibody adsorption chromatography. The final product was purified 20,000-fold over the starting material and was found to be free of any polypeptide contaminant as indicated by gel electrophoresis, sedimentation equilibrium ultracentrifugation, immunodiffusion, and immunoelectrophoresis.
UR - http://www.scopus.com/inward/record.url?scp=0019833088&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(81)90437-9
DO - 10.1016/0003-9861(81)90437-9
M3 - Article
C2 - 7305362
AN - SCOPUS:0019833088
SN - 0003-9861
VL - 211
SP - 119
EP - 124
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -