Characterization of lecithin:cholesterol acyltransferase expressed in a human lung cell line

Steven B. Lane, Kissaou T. Tchedre, Maya P. Nair, Anice E. Thigpen, Andras G. Lacko

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Lecithin:cholesterol acyltransferase (LCAT) is a key enzyme for the transfer of mammalian cholesterol from peripheral tissues to the liver. In patients deficient in LCAT, serum cholesterol levels rise and can lead to corneal opacity, proteinuria, anemia, and kidney failure. As early as 1968, relatively low volume transfusion of normal plasma was shown to temporarily correct the abnormal lipoprotein profiles in LCAT-deficient patients. However, despite the cloning, study, and extensive expression of LCAT in mammalian cell lines, there is still no viable, clinical therapy for LCAT deficiency. The current study was initiated to provide a source of recombinant human LCAT for enzyme replacement therapy. Accordingly, human LCAT has been cloned and expressed for the first time in a human cell line. The recombinant LCAT secreted by these cells was purified by phenyl-Sepharose chromatography, analyzed to determine the nature of its glycosylation, and tested for its enzymatic properties. The activity and basic kinetic parameters for the enzyme were determined using both a fluorescent water-soluble substrate and a macromolecular (proteoliposome) substrate. The enzymatic properties and the carbohydrate components of the recombinant LCAT were all sufficiently similar to those of the circulating human plasma enzyme, suggesting that this source of LCAT may be appropriate for use in some form of enzyme replacement therapy.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalProtein Expression and Purification
Volume36
Issue number2
DOIs
StatePublished - 1 Aug 2004

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Phosphatidylcholine-Sterol O-Acyltransferase
Cell Line
Lung
Enzyme Replacement Therapy
Lecithin Cholesterol Acyltransferase Deficiency
Enzymes
Cholesterol
Corneal Opacity
Agarose Chromatography
Glycosylation
Proteinuria
Lipoproteins
Renal Insufficiency
Organism Cloning
Anemia
Carbohydrates

Keywords

  • Human expression
  • Kinetic parameters
  • LCAT

Cite this

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title = "Characterization of lecithin:cholesterol acyltransferase expressed in a human lung cell line",
abstract = "Lecithin:cholesterol acyltransferase (LCAT) is a key enzyme for the transfer of mammalian cholesterol from peripheral tissues to the liver. In patients deficient in LCAT, serum cholesterol levels rise and can lead to corneal opacity, proteinuria, anemia, and kidney failure. As early as 1968, relatively low volume transfusion of normal plasma was shown to temporarily correct the abnormal lipoprotein profiles in LCAT-deficient patients. However, despite the cloning, study, and extensive expression of LCAT in mammalian cell lines, there is still no viable, clinical therapy for LCAT deficiency. The current study was initiated to provide a source of recombinant human LCAT for enzyme replacement therapy. Accordingly, human LCAT has been cloned and expressed for the first time in a human cell line. The recombinant LCAT secreted by these cells was purified by phenyl-Sepharose chromatography, analyzed to determine the nature of its glycosylation, and tested for its enzymatic properties. The activity and basic kinetic parameters for the enzyme were determined using both a fluorescent water-soluble substrate and a macromolecular (proteoliposome) substrate. The enzymatic properties and the carbohydrate components of the recombinant LCAT were all sufficiently similar to those of the circulating human plasma enzyme, suggesting that this source of LCAT may be appropriate for use in some form of enzyme replacement therapy.",
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Characterization of lecithin:cholesterol acyltransferase expressed in a human lung cell line. / Lane, Steven B.; Tchedre, Kissaou T.; Nair, Maya P.; Thigpen, Anice E.; Lacko, Andras G.

In: Protein Expression and Purification, Vol. 36, No. 2, 01.08.2004, p. 157-164.

Research output: Contribution to journalArticle

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