Characterization of bacterial diversity in drinking water by pyrosequencing

Yan Zhang, Qiang He

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Controlling microbial contamination of drinking water is critical to public health. However, understanding of the microbial ecology of drinking water remains incomplete. Representing the first application of high-throughput sequencing in drinking water microbiology, the objective of this study is to evaluate pyrosequencing as a high-throughput technique for the characterization of bacterial diversity in drinking water in comparison with conventional clone library analysis. Pyrosequencing and clone library analysis were performed in parallel to study the bacterial community composition in drinking water samples following the concentration of microbial biomass in drinking water with ultrafiltration. Validated by clone library analysis, pyrosequencing was confirmed as a highly efficient deep-sequencing technique to characterize the bacterial diversity in drinking water. Sequences of Alphaproteobacteria and Betaproteobacteria dominated the bacterial community in drinking water with Oxalobacteraceae and Methylobacteriaceae as the most abundant bacterial families, which is consistent with the prominent abundance of these populations frequently detected in various freshwater environments where source waters originate. Bacterial populations represented by the most abundant sequences in drinking water were closely related to cultures of metabolically versatile bacterial taxa widely distributed in the environment, suggesting a potential link between environmental distribution, metabolic characteristics, and abundance in drinking water.

Original languageEnglish
Pages (from-to)358-367
Number of pages10
JournalWater Science and Technology: Water Supply
Volume13
Issue number2
DOIs
StatePublished - 1 Jan 2013

Fingerprint

drinking water
clone
microbial ecology
freshwater environment
microbiology
ultrafiltration
community composition
public health
biomass
analysis
library

Keywords

  • Diversity
  • Drinking water
  • Microbial ecology
  • Proteobacteria
  • Pyrosequencing
  • Ultrafiltration

Cite this

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Characterization of bacterial diversity in drinking water by pyrosequencing. / Zhang, Yan; He, Qiang.

In: Water Science and Technology: Water Supply, Vol. 13, No. 2, 01.01.2013, p. 358-367.

Research output: Contribution to journalArticleResearchpeer-review

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