TY - JOUR
T1 - Characterization of a spontaneously immortalized bovine trabecular meshwork cell line.
AU - Mao, Weiming
AU - Liu, Yang
AU - Mody, Avani
AU - Montecchi-Palmer, Michela
AU - Wordinger, Robert J.
AU - Clark, Abbot F.
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2012/12
Y1 - 2012/12
N2 - Trabecular meshwork (TM) cells have widely been used as an in vitro model for glaucoma research. However, primary TM cells suffer the disadvantages of limited cell numbers and slow rates of proliferation. We discovered a spontaneously transformed bovine TM (BTM) cell line, BTM-28T. This cell line proliferated rapidly in low-glucose culture medium but also demonstrated contact inhibition in high-glucose culture medium. BTM-28T cells expressed key TM cell markers including α-smooth muscle actin (α-SMA), laminin and collagen IV (col IV). Also, 100 nM dexamethasone (DEX) enhanced the formation of cross-linked actin networks (CLANs) in confluent BTM-28T cell cultures. Transforming growth factor beta 2 (TGFβ2) induced the expression of fibronectin (FN), plasminogen activator inhibitor-1 (PAI-1), and connective tissue growth factor (CTGF) in our cell cultures. This cell line will be helpful to better understand the aqueous humor outflow pathway as related to the pathophysiology of glaucoma.
AB - Trabecular meshwork (TM) cells have widely been used as an in vitro model for glaucoma research. However, primary TM cells suffer the disadvantages of limited cell numbers and slow rates of proliferation. We discovered a spontaneously transformed bovine TM (BTM) cell line, BTM-28T. This cell line proliferated rapidly in low-glucose culture medium but also demonstrated contact inhibition in high-glucose culture medium. BTM-28T cells expressed key TM cell markers including α-smooth muscle actin (α-SMA), laminin and collagen IV (col IV). Also, 100 nM dexamethasone (DEX) enhanced the formation of cross-linked actin networks (CLANs) in confluent BTM-28T cell cultures. Transforming growth factor beta 2 (TGFβ2) induced the expression of fibronectin (FN), plasminogen activator inhibitor-1 (PAI-1), and connective tissue growth factor (CTGF) in our cell cultures. This cell line will be helpful to better understand the aqueous humor outflow pathway as related to the pathophysiology of glaucoma.
UR - http://www.scopus.com/inward/record.url?scp=84873165768&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2012.10.007
DO - 10.1016/j.exer.2012.10.007
M3 - Article
C2 - 23116564
AN - SCOPUS:84873165768
SN - 0014-4835
VL - 105
SP - 53
EP - 59
JO - Experimental Eye Research
JF - Experimental Eye Research
ER -