TY - JOUR
T1 - Characterization of a murine pressure ulcer model to assess efficacy of adipose-derived stromal cells
AU - Strong, Amy L.
AU - Bowles, Annie C.
AU - MacCrimmon, Connor P.
AU - Lee, Stephen J.
AU - Frazier, Trivia P.
AU - Katz, Adam J.
AU - Gawronska-Kozak, Barbara
AU - Bunnell, Bruce A.
AU - Gimble, Jeffrey M.
N1 - Funding Information:
Disclosure: Dr. Gimble is the co-owner, co-founder, and Chief Scientific Officer of LaCell LLC. Drs. Katz and Gawronska-Kozak are consultants to LaCell LLC. None of the other authors has any financial disclosures. This study was supported by the National Institute on Aging of the National Institutes of Health under award number R43AG043904, and funds from this grant was used to pay for the Article Processing Charge.
Publisher Copyright:
© 2015 The Authors.
PY - 2015
Y1 - 2015
N2 - Background: As the world's population lives longer, the number of individuals at risk for pressure ulcers will increase considerably in the coming decades. In developed countries, up to 18% of nursing home residents suffer from pressure ulcers and the resulting hospital costs can account for up to 4% of a nation's health care budget. Although full-thickness surgical skin wounds have been used as a model, preclinical rodent studies have demonstrated that repeated cycles of ischemia and reperfusion created by exposure to magnets most closely mimic the human pressure ulcer condition. Methods: This study uses in vivo and in vitro quantitative parameters to characterize the temporal kinetics and histology of pressure ulcers in young, female C57BL/6 mice exposed to 2 or 3 ischemia-reperfusion cycles. This pressure ulcer model was validated further in studies examining the efficacy of adipose-derived stromal/stem cell administration. Results: Optimal results were obtained with the 2-cycle model based on the wound size, histology, and gene expression profile of representative angiogenic and reparative messenger RNAs. When treated with adipose-derived stromal/stem cells, pressure ulcer wounds displayed a dose-dependent and significant acceleration in wound closure rates and improved tissue histology. Conclusion: These findings document the utility of this simplified preclinical model for the evaluation of novel tissue engineering and medical approaches to treat pressure ulcers in humans.
AB - Background: As the world's population lives longer, the number of individuals at risk for pressure ulcers will increase considerably in the coming decades. In developed countries, up to 18% of nursing home residents suffer from pressure ulcers and the resulting hospital costs can account for up to 4% of a nation's health care budget. Although full-thickness surgical skin wounds have been used as a model, preclinical rodent studies have demonstrated that repeated cycles of ischemia and reperfusion created by exposure to magnets most closely mimic the human pressure ulcer condition. Methods: This study uses in vivo and in vitro quantitative parameters to characterize the temporal kinetics and histology of pressure ulcers in young, female C57BL/6 mice exposed to 2 or 3 ischemia-reperfusion cycles. This pressure ulcer model was validated further in studies examining the efficacy of adipose-derived stromal/stem cell administration. Results: Optimal results were obtained with the 2-cycle model based on the wound size, histology, and gene expression profile of representative angiogenic and reparative messenger RNAs. When treated with adipose-derived stromal/stem cells, pressure ulcer wounds displayed a dose-dependent and significant acceleration in wound closure rates and improved tissue histology. Conclusion: These findings document the utility of this simplified preclinical model for the evaluation of novel tissue engineering and medical approaches to treat pressure ulcers in humans.
UR - http://www.scopus.com/inward/record.url?scp=84988935957&partnerID=8YFLogxK
U2 - 10.1097/GOX.0000000000000260
DO - 10.1097/GOX.0000000000000260
M3 - Article
AN - SCOPUS:84988935957
SN - 2169-7574
VL - 3
JO - Plastic and Reconstructive Surgery - Global Open
JF - Plastic and Reconstructive Surgery - Global Open
IS - 3
M1 - e334
ER -