@inbook{151c3da7a8d342e0806064998ecfa1bf,
title = "Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs",
abstract = "Protein kinase C (PKC), a family of serine/threonine kinases, plays an important role in apoptosis. Several members of the PKC family act as substrates for caspases. In addition, PKCs can also regulate caspase activation and cell death by apoptosis. The cleavage of PKCs separates the regulatory domain from the catalytic domain. The full-length, the catalytic domain, and the regulatory domain of PKC family members may have distinct function in apoptosis. Delineating the role of protein kinase C (PKC) isozymes in apoptosis has been challenging because of the lack of selective inhibitors of PKC isozymes and difficulty in generating stable cell lines expressing pro-apoptotic PKC isozymes. In this chapter, we describe the use of RNA interference (siRNA) technology and tetracycline-inducible expression of PKC isozymes to study their function in apoptosis.",
author = "Alakananda Basu and Persaud, {Shalini D.} and Usha Sivaprasad",
note = "Funding Information: The authors wish to acknowledge Dr. Baohua Sun for the cloning of PKCδ and Ms. Jie Huang for the generation of the PKCδ Tet‐inducible system. This work was supported by grants CA71727 and CA85682 from the National Cancer Institute.",
year = "2008",
doi = "10.1016/S0076-6879(08)01608-X",
language = "English",
isbn = "9780123744647",
series = "Methods in Enzymology",
pages = "141--157",
editor = "Roya Khosravi-Far and Zahra Zakeri and Richard Lockshin and Mauro Piacentini",
booktitle = "Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B",
}