Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs

Alakananda Basu; Shalini D. Persaud; Usha Sivaprasad

doi:10.1016/S0076-6879(08)01608-X

Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs

Alakananda Basu, Shalini D. Persaud, Usha Sivaprasad

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

Protein kinase C (PKC), a family of serine/threonine kinases, plays an important role in apoptosis. Several members of the PKC family act as substrates for caspases. In addition, PKCs can also regulate caspase activation and cell death by apoptosis. The cleavage of PKCs separates the regulatory domain from the catalytic domain. The full-length, the catalytic domain, and the regulatory domain of PKC family members may have distinct function in apoptosis. Delineating the role of protein kinase C (PKC) isozymes in apoptosis has been challenging because of the lack of selective inhibitors of PKC isozymes and difficulty in generating stable cell lines expressing pro-apoptotic PKC isozymes. In this chapter, we describe the use of RNA interference (siRNA) technology and tetracycline-inducible expression of PKC isozymes to study their function in apoptosis.

Original language	English
Title of host publication	Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B
Editors	Roya Khosravi-Far, Zahra Zakeri, Richard Lockshin, Mauro Piacentini
Pages	141-157
Number of pages	17
DOIs	https://doi.org/10.1016/S0076-6879(08)01608-X
State	Published - 2008

Publication series

Name	Methods in Enzymology
Volume	446
ISSN (Print)	0076-6879

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10.1016/S0076-6879(08)01608-X

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Basu, A., Persaud, S. D., & Sivaprasad, U. (2008). Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs. In R. Khosravi-Far, Z. Zakeri, R. Lockshin, & M. Piacentini (Eds.), Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B (pp. 141-157). (Methods in Enzymology; Vol. 446). https://doi.org/10.1016/S0076-6879(08)01608-X

Basu, Alakananda ; Persaud, Shalini D. ; Sivaprasad, Usha. / Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs. Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B. editor / Roya Khosravi-Far ; Zahra Zakeri ; Richard Lockshin ; Mauro Piacentini. 2008. pp. 141-157 (Methods in Enzymology).

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title = "Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs",

abstract = "Protein kinase C (PKC), a family of serine/threonine kinases, plays an important role in apoptosis. Several members of the PKC family act as substrates for caspases. In addition, PKCs can also regulate caspase activation and cell death by apoptosis. The cleavage of PKCs separates the regulatory domain from the catalytic domain. The full-length, the catalytic domain, and the regulatory domain of PKC family members may have distinct function in apoptosis. Delineating the role of protein kinase C (PKC) isozymes in apoptosis has been challenging because of the lack of selective inhibitors of PKC isozymes and difficulty in generating stable cell lines expressing pro-apoptotic PKC isozymes. In this chapter, we describe the use of RNA interference (siRNA) technology and tetracycline-inducible expression of PKC isozymes to study their function in apoptosis.",

author = "Alakananda Basu and Persaud, {Shalini D.} and Usha Sivaprasad",

note = "Funding Information: The authors wish to acknowledge Dr. Baohua Sun for the cloning of PKCδ and Ms. Jie Huang for the generation of the PKCδ Tet‐inducible system. This work was supported by grants CA71727 and CA85682 from the National Cancer Institute.",

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language = "English",

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series = "Methods in Enzymology",

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Basu, A, Persaud, SD & Sivaprasad, U 2008, Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs. in R Khosravi-Far, Z Zakeri, R Lockshin & M Piacentini (eds), Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B. Methods in Enzymology, vol. 446, pp. 141-157. https://doi.org/10.1016/S0076-6879(08)01608-X

Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs. / Basu, Alakananda; Persaud, Shalini D.; Sivaprasad, Usha.

Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B. ed. / Roya Khosravi-Far; Zahra Zakeri; Richard Lockshin; Mauro Piacentini. 2008. p. 141-157 (Methods in Enzymology; Vol. 446).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Persaud, Shalini D.

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N1 - Funding Information: The authors wish to acknowledge Dr. Baohua Sun for the cloning of PKCδ and Ms. Jie Huang for the generation of the PKCδ Tet‐inducible system. This work was supported by grants CA71727 and CA85682 from the National Cancer Institute.

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AB - Protein kinase C (PKC), a family of serine/threonine kinases, plays an important role in apoptosis. Several members of the PKC family act as substrates for caspases. In addition, PKCs can also regulate caspase activation and cell death by apoptosis. The cleavage of PKCs separates the regulatory domain from the catalytic domain. The full-length, the catalytic domain, and the regulatory domain of PKC family members may have distinct function in apoptosis. Delineating the role of protein kinase C (PKC) isozymes in apoptosis has been challenging because of the lack of selective inhibitors of PKC isozymes and difficulty in generating stable cell lines expressing pro-apoptotic PKC isozymes. In this chapter, we describe the use of RNA interference (siRNA) technology and tetracycline-inducible expression of PKC isozymes to study their function in apoptosis.

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Chapter 8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs

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