TY - JOUR
T1 - Changes in plasma apo B, apo E, apo A-1, and apo A-IV concentrations in dogs consuming different atherogenic diets
AU - Melchior, George W.
AU - Dory, Ladislav
AU - Roheim, Paul S.
PY - 1984/7
Y1 - 1984/7
N2 - To define the relationship between apoprotein levels and plasma cholesterol concentration in dogs, we measured the cholesterol, apo B, apo E, apo A-IV, and apo A-1 levels in 6 dogs fed a synthetic diet (Diet I), and in 5 dogs fed dog chow supplemented with lard, cholesterol, bile salts, and propylthiouracil (Diet II). The diet-induced hypercholesterolemia exceeded 900 mg/dl in dogs fed Diet I and was accompanied by a 12-fold increase in apo B, a 30-fold increase in apo E, an 8-fold increase in apo A-IV, and a l2'-fold increase in apo A-1. By contrast, the hypercholesterolemia averaged 1300 mg/dl in dogs fed Diet II and was accompanied by a 12-fold increase in apo B, an 11-fold increase in apo E, a 3-fold increase in apo A-IV, and a 5-fold decrease in apo A-1 levels. When 3 of the Diet I dogs were switched to dog chow, their plasma cholesterol, apo B, and apo E levels dropped to 30% of their peak value within 7 days. The change in apo B and apo E levels was found to be highly correlated with the change in plasma cholesterol concentrations in each of the Diet I animals (r2 ranged from 0.92 to 0.99 for both apoproteins). A strong linear relationship was also observed between apo E and apo B (r2 ranged from 0.94 to 0.98), indicating that the plasma apo E to apo B ratio remained constant in these animals as the hypercholesterolemia progressed or regressed.
AB - To define the relationship between apoprotein levels and plasma cholesterol concentration in dogs, we measured the cholesterol, apo B, apo E, apo A-IV, and apo A-1 levels in 6 dogs fed a synthetic diet (Diet I), and in 5 dogs fed dog chow supplemented with lard, cholesterol, bile salts, and propylthiouracil (Diet II). The diet-induced hypercholesterolemia exceeded 900 mg/dl in dogs fed Diet I and was accompanied by a 12-fold increase in apo B, a 30-fold increase in apo E, an 8-fold increase in apo A-IV, and a l2'-fold increase in apo A-1. By contrast, the hypercholesterolemia averaged 1300 mg/dl in dogs fed Diet II and was accompanied by a 12-fold increase in apo B, an 11-fold increase in apo E, a 3-fold increase in apo A-IV, and a 5-fold decrease in apo A-1 levels. When 3 of the Diet I dogs were switched to dog chow, their plasma cholesterol, apo B, and apo E levels dropped to 30% of their peak value within 7 days. The change in apo B and apo E levels was found to be highly correlated with the change in plasma cholesterol concentrations in each of the Diet I animals (r2 ranged from 0.92 to 0.99 for both apoproteins). A strong linear relationship was also observed between apo E and apo B (r2 ranged from 0.94 to 0.98), indicating that the plasma apo E to apo B ratio remained constant in these animals as the hypercholesterolemia progressed or regressed.
KW - Apo A-I
KW - Apo A-IV
KW - Apo B
KW - Apo E
KW - Hypercholesterolemia
UR - http://www.scopus.com/inward/record.url?scp=0021244045&partnerID=8YFLogxK
U2 - 10.1016/0021-9150(84)90155-2
DO - 10.1016/0021-9150(84)90155-2
M3 - Article
C2 - 6432002
AN - SCOPUS:0021244045
SN - 0021-9150
VL - 52
SP - 47
EP - 57
JO - Atherosclerosis
JF - Atherosclerosis
IS - 1
ER -