Ceruloplasmin, a Potential Therapeutic Agent for Alzheimer's Disease

Ya Shuo Zhao, Li Hong Zhang, Pan Pan Yu, Yu Jing Gou, Jing Zhao, Lin Hao You, Zhan You Wang, Xin Zheng, Liang-Jun Yan, Peng Yu, Yan Zhong Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aims: Ceruloplasmin (CP), a ferrous oxidase enzyme, plays an important role in regulating iron metabolism and redox reactions. Previous studies showed that CP deficiency contributes to Parkinson's disease by increasing iron accumulation and oxidative stress in the substantia nigra. However, the role of CP in Alzheimer's disease (AD) is unclear. We hypothesized that the lack of CP gene expression would affect the pathogenesis and damage of AD by promoting abnormal iron levels and oxidative stress. Results: AD mouse models were induced in CP knockout mouse either by injection of Aβ 25-35 into the lateral ventricle of the brain or transgenic APP expression. CP levels were decreased significantly in the hippocampus of AD patients, as well as Aβ-CP +/+ and APP-CP +/+ mice. Compared to control AD mice, CP gene deletion increased memory impairment and iron accumulation, which could be associated with elevated reactive oxygen species (ROS) levels and lead to cell apoptosis mediated through the Bcl-2/Bax and Erk/p38 signaling pathways in Aβ-CP -/- and APP-CP -/- mice. In contrast, the restoration of CP expression to CP -/- mice through injection of an exogenous expression plasmid into the brain ventricle alleviated Aβ-induced neuronal damage in the hippocampus. Innovation: CP alterations in iron contents were mediated through DMT1(-IRE) and changes in ROS levels, which in turn attenuated the progression of AD through the Erk/p38 and Bcl-2/Bax signaling pathways. Conclusion: Our results show a protective role of CP in AD and suggest that regulating CP expression in the hippocampus may provide a new neuroprotective strategy for AD. Antioxid. Redox Signal. 28, 1323-1337.

Original languageEnglish
Pages (from-to)1323-1337
Number of pages15
JournalAntioxidants and Redox Signaling
Volume28
Issue number14
DOIs
StatePublished - 10 May 2018

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Ceruloplasmin
Alzheimer Disease
Therapeutics
Iron
Hippocampus
Oxidative stress
Oxidation-Reduction
Reactive Oxygen Species
Oxidative Stress
Brain
Disease control
Injections
Lateral Ventricles
Gene Deletion
Redox reactions
Substantia Nigra
Knockout Mice
Metabolism
Gene expression

Keywords

  • Alzheimer's disease
  • apoptosis
  • ceruloplasmin
  • iron
  • oxidative stress
  • β-amyloid

Cite this

Zhao, Y. S., Zhang, L. H., Yu, P. P., Gou, Y. J., Zhao, J., You, L. H., ... Chang, Y. Z. (2018). Ceruloplasmin, a Potential Therapeutic Agent for Alzheimer's Disease. Antioxidants and Redox Signaling, 28(14), 1323-1337. https://doi.org/10.1089/ars.2016.6883
Zhao, Ya Shuo ; Zhang, Li Hong ; Yu, Pan Pan ; Gou, Yu Jing ; Zhao, Jing ; You, Lin Hao ; Wang, Zhan You ; Zheng, Xin ; Yan, Liang-Jun ; Yu, Peng ; Chang, Yan Zhong. / Ceruloplasmin, a Potential Therapeutic Agent for Alzheimer's Disease. In: Antioxidants and Redox Signaling. 2018 ; Vol. 28, No. 14. pp. 1323-1337.
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abstract = "Aims: Ceruloplasmin (CP), a ferrous oxidase enzyme, plays an important role in regulating iron metabolism and redox reactions. Previous studies showed that CP deficiency contributes to Parkinson's disease by increasing iron accumulation and oxidative stress in the substantia nigra. However, the role of CP in Alzheimer's disease (AD) is unclear. We hypothesized that the lack of CP gene expression would affect the pathogenesis and damage of AD by promoting abnormal iron levels and oxidative stress. Results: AD mouse models were induced in CP knockout mouse either by injection of Aβ 25-35 into the lateral ventricle of the brain or transgenic APP expression. CP levels were decreased significantly in the hippocampus of AD patients, as well as Aβ-CP +/+ and APP-CP +/+ mice. Compared to control AD mice, CP gene deletion increased memory impairment and iron accumulation, which could be associated with elevated reactive oxygen species (ROS) levels and lead to cell apoptosis mediated through the Bcl-2/Bax and Erk/p38 signaling pathways in Aβ-CP -/- and APP-CP -/- mice. In contrast, the restoration of CP expression to CP -/- mice through injection of an exogenous expression plasmid into the brain ventricle alleviated Aβ-induced neuronal damage in the hippocampus. Innovation: CP alterations in iron contents were mediated through DMT1(-IRE) and changes in ROS levels, which in turn attenuated the progression of AD through the Erk/p38 and Bcl-2/Bax signaling pathways. Conclusion: Our results show a protective role of CP in AD and suggest that regulating CP expression in the hippocampus may provide a new neuroprotective strategy for AD. Antioxid. Redox Signal. 28, 1323-1337.",
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Zhao, YS, Zhang, LH, Yu, PP, Gou, YJ, Zhao, J, You, LH, Wang, ZY, Zheng, X, Yan, L-J, Yu, P & Chang, YZ 2018, 'Ceruloplasmin, a Potential Therapeutic Agent for Alzheimer's Disease', Antioxidants and Redox Signaling, vol. 28, no. 14, pp. 1323-1337. https://doi.org/10.1089/ars.2016.6883

Ceruloplasmin, a Potential Therapeutic Agent for Alzheimer's Disease. / Zhao, Ya Shuo; Zhang, Li Hong; Yu, Pan Pan; Gou, Yu Jing; Zhao, Jing; You, Lin Hao; Wang, Zhan You; Zheng, Xin; Yan, Liang-Jun; Yu, Peng; Chang, Yan Zhong.

In: Antioxidants and Redox Signaling, Vol. 28, No. 14, 10.05.2018, p. 1323-1337.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ceruloplasmin, a Potential Therapeutic Agent for Alzheimer's Disease

AU - Zhao, Ya Shuo

AU - Zhang, Li Hong

AU - Yu, Pan Pan

AU - Gou, Yu Jing

AU - Zhao, Jing

AU - You, Lin Hao

AU - Wang, Zhan You

AU - Zheng, Xin

AU - Yan, Liang-Jun

AU - Yu, Peng

AU - Chang, Yan Zhong

PY - 2018/5/10

Y1 - 2018/5/10

N2 - Aims: Ceruloplasmin (CP), a ferrous oxidase enzyme, plays an important role in regulating iron metabolism and redox reactions. Previous studies showed that CP deficiency contributes to Parkinson's disease by increasing iron accumulation and oxidative stress in the substantia nigra. However, the role of CP in Alzheimer's disease (AD) is unclear. We hypothesized that the lack of CP gene expression would affect the pathogenesis and damage of AD by promoting abnormal iron levels and oxidative stress. Results: AD mouse models were induced in CP knockout mouse either by injection of Aβ 25-35 into the lateral ventricle of the brain or transgenic APP expression. CP levels were decreased significantly in the hippocampus of AD patients, as well as Aβ-CP +/+ and APP-CP +/+ mice. Compared to control AD mice, CP gene deletion increased memory impairment and iron accumulation, which could be associated with elevated reactive oxygen species (ROS) levels and lead to cell apoptosis mediated through the Bcl-2/Bax and Erk/p38 signaling pathways in Aβ-CP -/- and APP-CP -/- mice. In contrast, the restoration of CP expression to CP -/- mice through injection of an exogenous expression plasmid into the brain ventricle alleviated Aβ-induced neuronal damage in the hippocampus. Innovation: CP alterations in iron contents were mediated through DMT1(-IRE) and changes in ROS levels, which in turn attenuated the progression of AD through the Erk/p38 and Bcl-2/Bax signaling pathways. Conclusion: Our results show a protective role of CP in AD and suggest that regulating CP expression in the hippocampus may provide a new neuroprotective strategy for AD. Antioxid. Redox Signal. 28, 1323-1337.

AB - Aims: Ceruloplasmin (CP), a ferrous oxidase enzyme, plays an important role in regulating iron metabolism and redox reactions. Previous studies showed that CP deficiency contributes to Parkinson's disease by increasing iron accumulation and oxidative stress in the substantia nigra. However, the role of CP in Alzheimer's disease (AD) is unclear. We hypothesized that the lack of CP gene expression would affect the pathogenesis and damage of AD by promoting abnormal iron levels and oxidative stress. Results: AD mouse models were induced in CP knockout mouse either by injection of Aβ 25-35 into the lateral ventricle of the brain or transgenic APP expression. CP levels were decreased significantly in the hippocampus of AD patients, as well as Aβ-CP +/+ and APP-CP +/+ mice. Compared to control AD mice, CP gene deletion increased memory impairment and iron accumulation, which could be associated with elevated reactive oxygen species (ROS) levels and lead to cell apoptosis mediated through the Bcl-2/Bax and Erk/p38 signaling pathways in Aβ-CP -/- and APP-CP -/- mice. In contrast, the restoration of CP expression to CP -/- mice through injection of an exogenous expression plasmid into the brain ventricle alleviated Aβ-induced neuronal damage in the hippocampus. Innovation: CP alterations in iron contents were mediated through DMT1(-IRE) and changes in ROS levels, which in turn attenuated the progression of AD through the Erk/p38 and Bcl-2/Bax signaling pathways. Conclusion: Our results show a protective role of CP in AD and suggest that regulating CP expression in the hippocampus may provide a new neuroprotective strategy for AD. Antioxid. Redox Signal. 28, 1323-1337.

KW - Alzheimer's disease

KW - apoptosis

KW - ceruloplasmin

KW - iron

KW - oxidative stress

KW - β-amyloid

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